Toll样受体2及其信号通路在大鼠面神经夹挫伤后的机制研究  

The mechanism of TLR-2 and signal channel after facial nerve crush injury in rats

作　　者：李立恒 远洋[2] LI Liheng;YUAN Yang(Department of Otolaryngology,Huzhou Central Hospital(Central Hospital Affiliated to Huzhou Normal University),Huzhou,Zhejiang,313099,China;Department of Otolaryngology,Affiliated Hospital of Weifang Medical University,Weifang Shandong,261000,China)

机构地区：[1]湖州市中心医院(湖州师范学院附属中心医院)耳鼻咽喉科,浙江湖州313099 [2]潍坊医学院附属医院耳鼻咽喉科,山东潍坊261000

出　　处：《中国耳鼻咽喉头颈外科》2023年第10期629-632,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：湖州市中心医院博士启动基金(KY-BSQD-202307);湖州市科技局项目(2021GYB36);潍坊医学院附属医院博士启动基金(2022BSQD08)。

摘　　要：目的探究面神经损伤对大鼠Toll样受体2(TLR2)/核转录因子κB(NF-κB)信号通路的影响。方法将面神经损伤大鼠随机分为三组,A组:正常对照组,B组:手术损伤后自然恢复的观察组,C组:手术后腹腔注射Pam3CSK4(TLR2激动剂)观察恢复。电镜比较3组面神经的表现;HE染色脑干组织观察神经元的凋亡,PCR观察TLR2、NF-κB p65 mRNA表达水平,免疫荧光观察TLR2、NF-κB的荧光表达。结果面瘫造模顺利,电镜下面神经损伤明显,C组大鼠髓鞘较B组更为破碎;脑区内神经元也表现为胶质细胞变性更多;PCR显示A组TLR2及NF-κB p65 mRNA表达低于B组和C组;免疫荧光显示B组表达的TLR2、NF-κB荧光强于A组。结论注射Pam3CSK4激活TLR2/NF-κB信号通路,加重炎症的表现使面神经功能障碍。OBJECTIVE To investigate the effect of facial nerve injury on toll-like receptor 2(TLR2)/nuclear transcription factor-kappa B(NF-κB)signaling pathway in rats.METHODS The rats with facial nerve injury were randomly divided into three groups:normal control group A,group B was the observation group that recovered naturally after surgery injury,and group C was observed to recover after surgery by intrabitoneal injection of Pam3CSK4(TLR2 agonist).The manifestations of facial nerve in 3 groups were compared under electron microscope.Neuronal apoptosis was observed by HE staining,mRNA expression levels of TLR2 and NF-κB p65 were observed by PCR,and fluorescent expressions of TLR2 and NF-κB were observed by immunofluorescence.RESULTS Under electron microscope,nerve damage was obvious.Myelin sheath of rats in group C was more broken than that in group B.The neurons in the brain area also showed more degeneration of glial cells;PCR showed that the mRNA expressions of TLR2 and NF-κB p65 in group A were lower than those in group B and group C.Immunofluorescence showed that the fluorescence of TLR2 and NF-κB in group B was stronger than that in group A.CONCLUSION Injection of Pam3CSK4 activates the TLR2/NF-κB signaling pathway,aggravates the expression of inflammation and leads to facial nerve dysfunction.

关 键 词：TOLL样受体2 面神经损伤 核转录因子ΚB 

分 类 号：R745.12[医药卫生—神经病学与精神病学]