基于UPLC和HS-GC-MS的不同道地产区艾叶药材化学成分比较分析  

Comparison of chemical constituents in Artemisiae Argyi Folium from different Dao-di producing areas based on UPLC and HS-GC-MS

作　　者：王倩倩 郭蕊 张丹 郑玉光 郑倩[4] 郭龙 WANG Qian-qian;GUO Rui;ZHANG Dan;ZHENG Yu-guang;ZHENG Qian;GUO Long(Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province,Hebei University of Chinese Medicine,Shijiazhuang 050200,China;International Joint Research Center on Resource Utilization and Quality Evaluation of Traditional Chinese Medicine of Hebei Province,Hebei University of Chinese Medicine,Shijiazhuang 050200,China;Hebei Chemical and Pharmaceutical College,Shijiazhuang 050026,China;Shijiazhuang Hospital of Traditional Chinese Medicine,Shijiazhuang 050051,China)

机构地区：[1]河北中医药大学河北省中药炮制技术创新中心,河北石家庄050200 [2]河北中医药大学河北省中药资源利用与质量评价国际联合研究中心,河北石家庄050200 [3]河北化工医药职业技术学院,河北石家庄050026 [4]石家庄市中医院,河北石家庄050051

出　　处：《中国中药杂志》2023年第20期5509-5518,共10页China Journal of Chinese Materia Medica

基　　金：河北省自然科学基金项目(H2021423004,H2022418001);中央引导地方科技发展资金项目(226Z7714G);河北省省级科技计划项目(223777127D);河北省中医药管理局科研计划项目(Z2022019,2022100);河北省第二期现代农业产业技术体系创新团队项目(HBCT2018060205);第四次全国中药资源普查项目(Z135080000022)。

摘　　要：为了比较不同道地产区艾叶化学成分差异,共收集了3个主要道地产区(河北安国、河南南阳、湖北蕲春)共24批艾叶药材。采用超高效液相色谱法(UPLC)对艾叶药材中13个非挥发性成分的含量进行了测定和比较;进一步采用顶空气相色谱质谱联用法(HS-GC-MS)对艾叶药材中的挥发性成分进行定性分析和比较。结果表明艾叶非挥发性成分中酚酸类成分的含量明显高于黄酮类成分,但3个道地产区艾叶药材中非挥发性成分含量差异不明显;而3个道地产区艾叶药材中挥发性成分种类虽无显著差异,均含所鉴定出的40种挥发性成分,但在各挥发性成分相对含量上有显著差异;进一步运用主成分分析和偏最小二乘判别分析对不同道地产区艾叶的挥发性成分进行分析,共寻找到8个挥发性差异化合物,可以作为区分3个道地产区艾叶药材的指标性成分。通过以上研究,明确了不同道地产区艾叶药材的化学成分差异,为艾叶药材道地产地区分和质量评价提供了分析方法和科学依据。This study aims to compare the chemical constituents in 24 batches of Artemisiae Argyi Folium samples collected from three different Dao-di producing areas(Anguo in Hebei,Nanyang in Henan,and Qichun in Hubei).An ultra-performance liquid chromatography(UPLC) method was established to determine the content of 13 nonvolatile components,and headspace-gas chromatography-mass spectrometry(HS-GC-MS) was employed for qualitative analysis and comparison of the volatile components.The content of phenolic acids in Artemisiae Argyi Folium was higher than that of flavonoids,and the content of nonvolatile components showed no significant differences among the samples from the three Dao-di producing areas.A total of 40 volatile components were identified,and the relative content of volatile components in Artemisiae Argyi Folium was significantly different among the samples from different Dao-di producing areas.The principal component analysis and partial least squares discriminant analysis identified 8 volatile components as the potential markers for discrimination of Artemisiae Argyi Folium samples from different Dao-di producing areas.This study revealed the differences in the chemical composition of Artemisiae Argyi Folium samples from three different Dao-di producing areas,providing analytical methods and a scientific basis for the discrimination and quality evaluation of Artemisia Argyi Folium in different Dao-di producing areas.

关 键 词：艾叶 道地产区 非挥发性成分 挥发性成分 化学计量学 

分 类 号：O657.63[理学—分析化学] R284.1[理学—化学]