LILRB1和LILRB4在单核细胞分化急性髓系白血病诊断中的意义  

Significance of LILRB1 and LILRB4 in the diagnosis of monocyte-differentiated acute myeloid leukemia

作　　者：饶若[1] 王述文[1] 吴莉芳[1] 王照良 唐瑞梅[2] 林慧[3] RAO Ruo;WANG Shuwen;WU Lifang;WANG Zhaoliang;TANG Ruimei;LIN Hui(Clinical Laboratory,Hainan General Hospital,Hainan Affiliated Hospital of Hainan Medical University,Hainan Haikou 570311,China.;Department of Hematology,Hainan General Hospital,Hainan Affiliated Hospital of Hainan Medical University,Hainan Haikou 570311,China.;Department of Anesthesiology,Hainan General Hospital,Hainan Affiliated Hospital of Hainan Medical University,Hainan Haikou 570311,China)

机构地区：[1]海南省人民医院海南医学院附属海南医院检验科,海南海口570311 [2]海南省人民医院海南医学院附属海南医院血液科,海南海口570311 [3]海南省人民医院海南医学院附属海南医院麻醉科,海南海口570311

出　　处：《现代肿瘤医学》2023年第24期4582-4586,共5页Journal of Modern Oncology

基　　金：海南省自然科学基金资助项目(编号:821RC1113);海南省卫生健康行业科研项目(编号:21A200177);海南省临床医学中心建设项目资助[编号:琼卫医函[2022]341号]。

摘　　要：目的:探讨LILRB1和LILRB4在单核细胞分化急性髓系白血病(M-AML)诊断中的意义。方法:通过直接荧光标记流式细胞术检测109例急性白血病患者白血病细胞LILRB1、LILRB4、CD64和CD14的表达。结果:LILRB1在M-AML、NM-AML和ALL患者中表达阳性率分别为81.6%(31/38)、0(0/49)、27.3%(6/22),差异有统计学意义(P<0.01),其中M-AML组的阳性率明显高于NM-AML组和ALL组,ALL组阳性率明显高于NM-AML组;LILRB4在M-AML组中的表达阳性率为81.6%(31/38),在NM-AML和ALL组中均不表达(0/49,0/22),差异有统计学意义(P<0.01);单独检测LILRB1、LILRB4诊断M-AML敏感性均为81.6%,联合检测的敏感性为86.8%,特异性均为100%,优于CD64(86.8%,61.2%)、CD14(31.6%,100%)。结论:LILRB1、LILRB4单独检测或联合检测对检出M-AML均具有极高的灵敏度和特异度,可作为诊断M-AML的新指标。Objective:To explore the significance of LILRB1 and LILRB4 in the diagnosis of monocyte-differentiated acute myeloid leukemia(M-AML).Methods:Flow cytometry with direct immunofluorescence staining was used to detect the expression of LILRB1,LILRB4,CD64 and CD14 of 109 acute leukemia patients.Results:There was statistical difference in the positive rate of LILRB1 among M-AML,NM-AML and ALL(81.6%,0,27.3%,P<0.01).The positive rate of M-AML group was significantly higher than that of NM-AML group and ALL group,and the positive rate of ALL group was significantly higher than that of NM-AML group.The positive rate of LILRB4 in M-AML group was 81.6%(31/38),but LILRB4 was not expressed in both NM-AML and ALL groups(0/49,0/22),which has statistical difference(P<0.01).The sensitivity of individual detection of LILRB1 and LILRB4 in diagnosing M-AML was 81.6%,while the sensitivity of combined detection was 86.8%,and the specificity was 100%,which was better than CD64(86.8%,61.2%),CD14(31.6%,100%).Conclusion:Both LILRB1,LILRB4 alone or in combination have high sensitivity and specificity for detecting M-AML,which can be used as new indicators for the diagnosis of M-AML.

关 键 词：LILRB1 LILRB4 急性髓系白血病 单核细胞分化 流式细胞术 

分 类 号：R733.71[医药卫生—肿瘤]