CCNB2、Sox11及miR-21在子宫内膜癌中的表达及临床意义  

Expression of CCNB2,Sox11 and miR-21 in endometrial cancer and clinical significance

作　　者：封玲 史爱红 张蓓 FENG Ling;SHI Aihong;ZHANG Bei(Graduate School,Xuzhou Medical University,Jiangsu Xuzhou 221004,China;Department of Obstetrics and Gynecology,Shuyang Hospital Affiliated to Xuzhou Medical University,Jiangsu Shuyang 223600,China)

机构地区：[1]徐州医科大学研究生院,江苏徐州221004 [2]徐州医科大学附属沭阳医院妇产科,江苏沭阳223600

出　　处：《现代肿瘤医学》2023年第24期4601-4606,共6页Journal of Modern Oncology

摘　　要：目的:探讨细胞周期蛋白B2(CCNB2)、SRY-盒转录因子11(Sox11)及miR-21在子宫内膜癌中的表达及临床意义。方法:选择2015年1月至2017年12月诊治的子宫内膜癌患者(EMC组)及子宫内膜良性疾病患者(CON组)为研究对象,采用实时荧光定量PCR(RT-qPCR)检测CCNB2、Sox11及miR-21表达。采用蛋白质印迹法(Western blot)检测CCNB2、Sox11蛋白表达。比较不同临床病理因素中CCNB2、Sox11及miR-21表达的差异。采用ROC曲线分析血清CCNB2、Sox11及miR-21预测子宫内膜癌患者死亡效能。Logistic回归分析子宫内膜癌预后不良的危险因素。Kaplan-Meier法分析CCNB2、Sox11和miR-21表达与生存期的关系。结果:EMC组患者肿瘤组织中CCNB2、Sox11及miR-21相对表达量及CCNB2、Sox11蛋白表达均显著高于癌旁组织及CON组(均P<0.05)。Ishikawa、ECC-1、Hec50co和RL95-2子宫内膜癌细胞中CCNB2、Sox11及miR-21相对表达量及CCNB2、Sox11蛋白表达量均显著高于正常人子宫内膜上皮细胞HEMEC(均P<0.05)。EMC组患者肿瘤组织中CCNB2、Sox11及miR-21表达在分化程度、组织学分级、肌层浸润深度、淋巴结转移及FIGO分期中的差异有统计学意义(均P<0.05)。CCNB2、Sox11及miR-21表达预测子宫内膜癌患者死亡截断值分别为1.2、0.8、1.5,敏感度分别为0.771、0.827、0.833,特异性分别为0.785、0.762、0.701。低分化、组织学分级G3、肌层浸润深度>1/2、有淋巴结转移、III期、CCNB2相对表达量≥1.2、Sox11相对表达量≥0.8、miR-21相对表达量≥1.5为子宫内膜癌患者死亡的危险因素(P<0.05)。CCNB2表达≥1.2且Sox11表达≥0.8且miR-21表达≥1.5子宫内膜癌患者中位生存期显著低于CCNB2表达<1.2或Sox11表达<0.8或miR-21表达<1.5患者[中位生存期(27.2±6.4)月比(32.5±6.8)月,Logrank=9.821,P<0.05]。结论:子宫内膜癌患者CCNB2、Sox11及miR-21表达显著升高,与分化程度、组织学分级、肌层浸润深度、淋巴结转移及FIGO分期、生存期及�Objective:To investigate the expression of cyclin B2(CCNB2),SRY-box transcription factor 11(Sox11)and miR-21 in endometrial cancer and clinical significance.Methods:Patients with endometrial cancer(EMC group)and endometrial benign disease(CON group)who were diagnosed and treated from January 2015 to December 2017 were selected as research objects,and real-time fluorescent quantitative PCR(RT-qPCR)was used to detect CCNB2,Sox11 and miR-21 expression.Western blot was used to detect the protein expression of CCNB2 and Sox11.The differences in the expression of CCNB2,Sox11 and miR-21 in different clinicopathological factors were compared.The ROC curve was used to analyze the efficacy of serum CCNB2,Sox11 and miR-21 in predicting the death of patients with endometrial cancer.Logistic regression analysis evaluated the risk factors for poor prognosis in endometrial cancer.Kaplan-Meier method was used to analyze the relationship between the expression of CCNB2,Sox11 and miR-21 and the survival time.Results:The relative expressions of CCNB2,Sox11 and miR-21 in tumor tissues of EMC group were significantly higher than those in paracancerous tissues and CON group(all P<0.05).The relative expressions of CCNB2,Sox11 and miR-21 in Ishikawa,ECC-1,Hec50co and RL95-2 endometrial cancer cells were significantly higher than those in normal human endometrial epithelial cells HEMEC(all P<0.05).There were significant differences in the expression of CCNB2,Sox11 and miR-21 in the tumor tissue of EMC group with the degree of differentiation,histological grade,depth of myometrial invasion,lymph node metastasis and FIGO stage(all P<0.05).The cut-off values of CCNB2,Sox11 and miR-21 in predicting the death of endometrial cancer patients were 1.2,0.8,1.5.The sensitivities were 0.771,0.827,0.833,and the specificities were0.785,0.762,0.701,respectively.Poorly differentiated,histological grade G3,depth of myometrial invasion>1/2,lymph node metastasis,stageⅢ,relative expression of CCNB2≥1.2,relative expression of Sox11≥0.8,relative expres

关 键 词：子宫内膜癌 CCNB2 Sox11 MIR-21 临床价值 

分 类 号：R737.33[医药卫生—肿瘤]