吴茱萸碱通过GSK-3β/β-catenin通路减轻大鼠脑缺血再灌注损伤  

Evodiamine Attenuates Cerebral Ischemia-reperfusion Injury in Rats via the GSK-3β/β-Catenin Pathway

作　　者：赵晓华[1] 田倩倩[2] 薛伟 赵鑫 郭筱 刘君玲[2] Zhao Xiaohua;Tian Qianqian;Xue Wei;Zhao Xin;Guo Xiao;Liu Junling(Weifang Medical University,Weifang 261053,China;Department of Neurology,Affiliated Hospital of Weifang Medical University,Weifang 261031,China;Clinical Research Center,Affiliated Hospital of Weifang Medical University,Weifang 261041,China)

机构地区：[1]潍坊医学院,山东潍坊261053 [2]潍坊医学院附属医院神经内科,山东潍坊261031 [3]潍坊医学院附属医院临床医学研究中心,山东潍坊261041

出　　处：《南开大学学报（自然科学版）》2023年第5期23-28,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：山东省自然科学基金面上项目(ZR2020MH159);山东省中医药科技发展计划项目(2019-0423,2019-0412);潍坊医学院2022年度研究生科研创新基金项目;潍坊医学院附属医院临床研究项目(2022wyfylcyj01);潍坊市科学技术发展计划项目(医学类)(2020YX086)。

摘　　要：急性缺血性脑卒中超早期血管再通可挽救缺血半暗带,有时可出现缺血再灌注损伤,加重患者症状,探寻药物防治脑缺血再灌注损伤是研究热点.发现在脑缺血超早期4 h之内开始给予吴茱萸碱(Evo)连续应用3 d能有效改善脑缺血再灌注损伤的SD大鼠的神经功能评分,减轻皮质区神经元细胞损伤,增加Bcl-2蛋白表达水平,减少Bax、Caspase-3蛋白表达水平,调节GSK-3β/β-catenin信号传导通路,减轻神经元细胞凋亡及大鼠脑缺血再灌注损伤.结果表明,吴茱萸碱通过调控凋亡因子和GSK-3β/β-catenin信号通路减轻大鼠脑缺血再灌注损伤.Ultra-early vascular revascularization in acute ischemic stroke can rescue the ischemic penumbra,and sometimes ischemia-reperfusion injury can occur and aggravate the patient's symptoms,and it is a hot research topic to explore the drugs to prevent and treat cerebral ischemia-reperfusion injury.It is found that the administration of evodiamine(Evo)for 3 d within 4 h of cerebral ischemia can effectively improve the neurological function score of SD rats with cerebral ischemia-reperfusion injury,reduce neuronal cell injury in cortical areas,promote Bcl-2 protein expression level,reduce Bax and Caspase-3 protein expression level,upregulate GSK-3β/β-catenin signaling pathway,reduce neuronal cell apoptosis and cerebral ischemia-reperfusion injury in rats.The results showed that evodiamine attenuated ischemia-reperfusion injury in rat brains by regulating apoptotic factors and the GSK-3β/β-catenin signaling pathway.

关 键 词：吴茱萸碱 缺血再灌注 细胞凋亡 GSK-3β/β-catenin通路 

分 类 号：R743.32[医药卫生—神经病学与精神病学]