一株异养硝化-好氧反硝化菌株L3的筛选鉴定及其脱氮特性  

Screening and Identification of a Heterotrophic Nitrification-Aerobic Denitrification Strain L3 and its Nitrogen Removal Characteristics

作　　者：刘雨晴 张耀月 詹夏沁 李钰盈 陈源洲 陈琼华[1] LIU Yuqing;ZHANG Yaoyue;ZHAN Xiaqin;LI Yuying;CHEN Yuanzhou;CHEN Qionghua(School of Life Science,Guangzhou University,Guangzhou 510006,China)

机构地区：[1]广州大学生命科学学院,广东广州510006

出　　处：《水处理技术》2023年第12期94-100,共7页Technology of Water Treatment

基　　金：广东省自然科学基金(62004101);广东省科技计划项目(2019B030316022);中国—东盟渔业资源保护与开发利用(6911801);2020-2021年国家级大学生创新创业训练计划项目(202111078038)。

摘　　要：从广东三水罗非鱼养殖池底泥分离筛选出一株具有高效降氮能力的异养硝化-好氧反硝化细菌L3。通过形态学,生理生化以及16S rRNA基因序列分析鉴定该菌株为青岛假单胞菌Pseudomonas qingdaonensis。研究了该菌株对三种含氮模拟废水的脱氮特性及降氮过程的pH耐受范围,进一步采用响应面法探究了温度、pH、C/N、溶氧量及其交互作用对菌株L3脱氮性能的影响。结果表明,该菌株处理高浓度含氮模拟废水48 h后NH_(4)^(+)-N、NO-3-N、NO-2-N的去除率分别达到90.50%、65.24%、85.34%,且在酸性条件下(pH=4)有较强的降氮能力;响应面法结果表明菌株最佳脱氮条件为温度29.1℃,C/N为11.7,摇床转速175 r/min,pH为6.2,其中温度、碳氮比及二者之间的交互作用对NH_(4)^(+)-N去除率的影响显著;在最优条件下,菌株L3处理模拟氨氮废水12 h后对NH_(4)^(+)-N去除率可达95.50%,与预测值无显著差别,响应面模型可靠。A heterotrophic nitrification-aerobic denitrification bacteria L3 with high nitrogen removal ability was isolated from the sediment of a tilapia aquaculture pond in Sanshui,Guangdong.Based on morphological,biochemical and 16S rRNA gene sequence analysis,the strain was identified as Pseudomonas qingdaonensis.At the same time,the nitrogen removal ability of the strain for simulated nitrogen-containing wastewater and the pH tolerance range of the nitrogen removal process were studied.The response surface method was used to explore the effects of temperature,pH,C/N ratio,dissolved oxygen and their interactions on the nitrogen removal ability of strain L3.The results showed that the removal rates of NH_(4)^(+)-N,NO-3-N,NO-2-N reached 90.50%,65.24%and 85.34%respectively,after treating high-concentration nitrogen-containing simulated wastewater for 48 h.The strain L3 has a strong nitrogen removal ability under acidic conditions(pH=4).The optimum nitrogen removal conditions obtained by response surface methodology are as follows:temperature 29.1℃,C/N 11.7,shaking speed 175 r/min,pH 6.2.Among them,temperature,C/N ratio and the interaction between the two have significant effects on NH_(4)^(+)-N removal rate.Under the optimal conditions,the NH_(4)^(+)-N removal rate of strain L3 reached 95.50%after treating simulated ammonia nitrogen wastewater for 12 h,which was not significantly different from the predicted value,and the response surface model was reliable.

关 键 词：筛选 异养硝化 好氧反硝化 响应面法 生物脱氮 

分 类 号：X172[环境科学与工程—环境科学]