circPLOD2调节miR-217/ANLN轴对结肠癌细胞增殖、凋亡和免疫逃逸的影响  

Effects of circPLOD2 on the proliferation,apoptosis and immune escape of colon cancer by regulating miR-217/ANLN axis

作　　者：赵岭岭[1] 刘艳彩 李景光[1] 吴春平[1] 商庆花 ZHAO Ling-ling;LIU Yan-cai;LI Jing-guang;WU Chun-ping;SHANG Qing-hua(Department of Pathology,the No.4 People's Hospital of Hengshui,Hengshui 053099,China)

机构地区：[1]衡水市第四人民医院病理科,河北衡水053099

出　　处：《中国现代普通外科进展》2023年第11期852-856,共5页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:探讨circPLOD2对结肠癌细胞增殖、凋亡和免疫逃逸的影响以及对miR-217/ANLN轴的调节机制。方法:将HT-29细胞分为NC组、si-NC组、si-circPLOD2组、si-circPLOD2+anti-miR-NC组、si-circPLOD2+anti-miR-217组。qRT-PCR检测细胞中circPLOD2、miR-217、ANLN mRNA的表达;CCK-8法检测细胞增殖活性;流式细胞仪检测细胞凋亡;Western blot检测凋亡相关蛋白Bcl-2、Bax及PD-L1的表达;ELISA检测免疫逃逸相关因子TNF-α、IL-2、IFN-γ的水平;双荧光素酶报告基因实验验证circPLOD2、miR-217、ANLN三者的靶向关系。结果:与si-NC组相比,si-circPLOD2组HT-29细胞中circPLOD2、ANLN水平、细胞增殖活性、Bcl-2、PD-L1表达降低(P<0.05),miR-217水平、细胞凋亡率、Bax表达以及TNF-α、IL-2、IFN-γ水平升高(P<0.05),且双荧光素酶基因报告实验验证了circPLOD2、ANLN与miR-217之间均存在靶向关系。结论:敲低circPLOD2能够上调miR-217表达,下调ANLN的表达,抑制CC细胞的增殖和免疫逃逸,促进细胞凋亡。Objective:To investigate the effects of circPLOD2 on the proliferation,apoptosis and immune escape of colon cancer(CC)cells and its regulatory mechanism on the miR-217/ANLN axis in this process.Methods:HT-29 cells were divided into NC group,si-NC group,si-circPLOD2 group,si-circPLOD2+anti miR-NC group,and si-circPLOD2+anti miR-217 group.The expression of circPLOD2,miR-217 and ANLN mRNA was detected by qRT-PCR;cell proliferation activity was detected by CCK-8 method;apoptosis was detected by flow cytometry;Western blot was used to detect the expression of apoptosis related proteins Bcl-2,Bax and PD-L1;the levels of immune escape related factors TNF-α,IL-2 and IFN-γwere detected by ELISA;double luciferase reporter gene experiment was applied to verify the targeting relationship of circPLOD2,miR-217 and ANLN.Results:Compared with the si-NC group,the mRNA levels of circPLOD2 and ANLN,cell proliferation activity,the protein expression of Bcl-2 and PD-L1 in HT-29 cells of the si-circPLOD2 group decreased(P<0.05),the level of miR-217,apoptosis rate,the protein expression of Bax,and the levels of TNF-α,IL-2,and IFN-γincreased(P<0.05),and the double luciferase gene report experiment verified that there was a targeting relationship between circPLOD2,ANLN and miR-217.Conclusion:Knockdown circPLOD2 can up-regulate the expression of miR-217,down-regulate the expression of ANLN,inhibit the proliferation and immune escape of CC cells,and promote apoptosis.

关 键 词：circPLOD2 结肠癌 miR-217/ANLN轴 增殖 凋亡 免疫逃逸 

分 类 号：R735.35[医药卫生—肿瘤]