Ag-HA/TiO_(2)抗菌剂的制备及在大黄鱼中的应用  

Preparation of Ag-HA/TiO_(2) Antibacterial Agent and Application in Large Yellow Croaker

作　　者：李超群[1] 石娟 周丽萍[1] 王晴[1] 娄永江[1] LI Chaoqun;SHI Juan;ZHOU Liping;WANG Qing;LOU Yongjiang(College of Food and Pharmaceutical Sciences,Ningbo University,Ningbo 315832,China)

机构地区：[1]宁波大学食品与药学学院,浙江宁波315832

出　　处：《食品与生物技术学报》2023年第11期55-62,共8页Journal of Food Science and Biotechnology

基　　金：浙江省重点研发计划项目(2018C02025)。

摘　　要：为探究载银羟基磷灰石(AgHAp)/二氧化钛(Ag-HA/TiO_(2))抗菌剂在水产品中的应用,研究了Ag-HA/TiO_(2)的结构表征。选取大肠杆菌和金黄色葡萄球菌为测试菌株,比较了AgHAp和Ag-HA/TiO_(2)的抗菌性能,并初步判断Ag-HA/TiO_(2)对水产品特定腐败菌荧光假单胞菌和腐败希瓦氏菌(SSOs)的抗菌率,在此基础上进行了实际应用。测试了抗菌液质量浓度和浸泡时间对大黄鱼的微生物和一级鲜肉标准的影响,结果表明:Ag-HA/TiO_(2)负载二氧化钛、降低一半银质量分数后,结构晶形基本不变,Ag-HA/TiO_(2)对大肠杆菌和金黄色葡萄球菌的抗菌率为100%,比银质量分数为3.00%的AgHAp要好,且对该2种菌的最小抑菌质量浓度均为312.5μg/mL;10 mg/mL Ag-HA/TiO_(2)对SSOs在0.5 h时抑菌率分别为(97.58±3.43)%和100%,作用时间为1.0 h和1.5 h时均为100%;Ag-HA/TiO_(2)浸泡液质量浓度越高或大黄鱼浸泡时间越长,对大黄鱼中细菌生长越有明显的抑制效果,且延长大黄鱼一级鲜肉标准的货架期。该研究结果证明,Ag-HA/TiO_(2)抗菌具有高效性,有望成为一种理想的水产抗菌材料。In order to explore the application of silver-loaded hydroxyapatite/titanium dioxide(Ag-HA/TiO_(2))antibacterial agent in aquatic products,this study conducted the structural characterization of Ag-HA/TiO_(2) and selected Escherichia coli and Staphylococcus aureus as the test strains for a comparison between silver-loaded hydroxyapatite(AgHAp)and titanium dioxide(Ag-HA/TiO_(2))in antibacterial performance.A preliminary evaluation on the antibacterial rate of Ag-HA/TiO_(2) against specific spoilage bacteria(SSOs)in aquatic products was also assessed.Based on these evaluations,the study conducted practical applications to investigate the effects of the antibacterial solution concentration and the soaking time on the microorganisms in large yellow croaker and the standard for Grade I fresh meat.The results showed that Ag-HA/TiO_(2) maintained its crystalline structure after loading titanium dioxide and reducing silver content by half.The antibacterial rate of Ag-HA/TiO_(2) against Escherichia coli and Staphylococcus aureus was 100%,which was superior to AgHAp containing 3.00%of silver,and the minimum inhibitory concentration for both strains was 312.5μg/mL.The antibacterial rates of 10 mg/mL Ag-HA/TiO_(2) against SSOs-pseudomonas fluorescens and shewanella putrefaciens at 0.5 h were(97.58±3.43)%and 100%respectively,reaching 100%at 1.0 h and 1.5 h.As the Ag-HA/TiO_(2) was applied to large yellow croaker,higher concentration of soaking solution or longer soaking time resulted in more pronounced inhibition effect on the growth of bacteria in large yellow croaker,extending the standard shelf life for Grade I fresh meat of large yellow croaker.The study demonstrates the high antibacterial effectiveness of Ag-HA/TiO_(2) and suggests its potential as an ideal antibacterial agent for aquatic products.

关 键 词：载银羟基磷灰石 二氧化钛 结构表征 抗菌活性 

分 类 号：S983[农业科学—捕捞与储运]