燕窝罐头原料及生产中嗜热微生物分析  

Analysis of Thermophilic Microorganisms in Canned Bird’s Nest Production

作　　者：范力艺 范群艳 林佳馨 胡嘉淼 张怡[1] FAN Liyi;FAN Qunyan;LIN Jiaxin;HU Jiamiao;ZHANG Yi(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Xiamen Yanzhiwu Sinong Food Co.,Ltd.,Xiamen,360000,China;Cofeco Biotechnology Co.,Ltd.,Beijing 102200,China)

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]厦门燕之屋丝浓食品有限公司,福建厦门360000 [3]中粮生物科技股份有限公司,北京102200

出　　处：《食品与生物技术学报》2023年第11期88-97,共10页Journal of Food Science and Biotechnology

基　　金：福建省提升教育水平专项项目([2019]0761);福建省科技创新领军人才计划项目(KRC16002A)。

摘　　要：采用16S rRNA高通量测序技术结合传统培养分离方法,比较了燕窝罐头原料与不同生产工序的微生物群系构成,通过α、β微生物多样性分析,研究了燕窝罐头生产线中的腐败微生物污染源与工序的关键控制点。结果表明:冰糖原料是燕窝罐头的主要污染来源,其中携带的地芽孢杆菌属和芽孢杆菌属是燕窝罐头的主要腐败污染菌属。此外还确定了生产线上与冰糖原料相关的工序作为生产关键控制点,从而为燕窝罐头的生产质量控制和卫生监管提供理论依据。A combination of 16S rRNA high-throughput sequencing technology and traditional culture methods was used to analyze and compare the microbial communities in raw materials for canned bird's nest and different production processes.Meanwhile,αandβmicrobial diversity analyses were used to investigate key control points for microbial contamination sources and processes in the canned production lines.The results revealed that the primary source of contamination in canned bird's nest production was the raw material of rock sugar,and the Geobacillus and Bacillus were the main contaminant genus responsible for the spoilage of canned bird's nest.Furthermore,the production processes associated with rock sugar raw materials in the production line were identified as the production critical control points.This study provides a theoretical basis for the production quality control and hygiene supervision in canned bird's nest production.

关 键 词：燕窝 低酸性罐头 微生物多样性 污染 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]