多次补氮和增强蓝光促进三角褐指藻积累岩藻黄素  

Promoting fucoxanthin accumulation in Phaeodactylum tricornutum by multiple nitrogen supplementation and blue light enhancement

作　　者：杨泽雄 杨润青 诸德斐 魏东[1] YANG Zexiong;YANG Runqing;ZHU Defei;WEI Dong(School of Food Science and Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510640

出　　处：《生物工程学报》2023年第11期4580-4592,共13页Chinese Journal of Biotechnology

基　　金：广东省基础与应用基础研究基金重点项目(2019B1515120002)。

摘　　要：本研究旨在优化多次补氮和增强蓝光模式,以促进光发酵三角褐指藻(Phaeodactylum tricornutum)积累岩藻黄素。结果表明,在摇瓶中将含有胰蛋白胨和尿素的混合氮源(1:1,Nmol/Nmol;总氮浓度为0.02 mol/L)分6次加入培养系统是最佳的多次补氮模式;在5 L发酵罐中实施两阶段调光模式培养,在第二阶段增强蓝光(R:G:B=67.1:16.7:16.3)后,细胞密度、生物量生产率以及岩藻黄素的含量、产量和生产率分别达到1.12×10^(8)cells/mL、330mg/(d·L)、19.62mg/g、69.71mg/L和6.97mg/(d·L)。与红蓝光(R:G:B=70.9:18.3:10.9)下分6次补氮的一阶段培养相比,岩藻黄素含量显著提高了7.68%(P<0.05),但生产率无显著差异(P>0.05);与红蓝光(R:G:B=70.9:18.3:10.9)下一次性加入氮源的一阶段培养相比,岩藻黄素含量和生产率显著提高了45.98%和48.30%(P<0.05)。因此,本研究开发的多次补氮和增强蓝光的两阶段培养模式,有效促进了岩藻黄素积累、提高了氮源利用效率,为三角褐指藻光发酵生产岩藻黄素提供了新技术支撑。The aim of this study was to promote fucoxanthin accumulation in Phaeodactylum tricornutum by photo-fermentation through optimizing the mode of multiple nitrogen supplementation and blue light enhancement.The results showed that the mixed nitrogen source(tryptone:urea=1:1,N mol/N mol;total nitrogen concentration at 0.02 mol/L)added to the culture system by six times was the best mode in shake flasks.Two-phase culture with light adjustment was then carried out in 5 L photo-fermenter with an enhanced blue light(R:G:B=67.1:16.7:16.3)in the second phase,leading to improved cell density(1.12×10^(8) cells/mL),biomass productivity(330 mg/(d·L)),fucoxanthin content(19.62 mg/g),titer(69.71 mg/L)and productivity(6.97 mg/(d·L)).Compared with one-phase culture under red/blue(R:G:B=70.9:18.3:10.9)light and six-times nitrogen supplementation,the fucoxanthin content was significantly increased by 7.68%(P<0.05)but the productivity did not change significantly(P>0.05).Compared with onephase culture under red/blue(R:G:B=70.9:18.3:10.9)light and one-time nitrogen supplementation,the content and productivity of fucoxanthin were significantly increased by 45.98%and 48.30%(P<0.05),respectively.This study developed a two-phase culture mode with multiple nitrogen supplementation and blue light enhancement,which effectively promoted the accumulation of fucoxanthin and improved the efficiency of nitrogen source utilization,thus providing a new approach for fucoxanthin accumulation in P.tricornutum by photo-fermentation.

关 键 词：三角褐指藻 岩藻黄素 补氮 蓝光 两阶段培养 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]