北京市通州区食源性疾病监测病例中副溶血弧菌的病原特征分析  

Pathogenic characterization of Vibrio parahaemolyticus in foodborne disease surveillance cases in Tongzhou district,Beijing

作　　者：张萍[1] 王梦晗[2] 邹林[1] 甄博珺[1] 张扬[1] 张靖[1] 郭晓晨 高翔[1] ZHANG Ping;WANG Menghan;ZOU Lin;ZHEN Bojun;ZHANG Yang;ZHANG Jing;GUO Xiaochen;GAO Xiang(Tongzhou Center for Disease Control and Prevention,Beijing 101100,China;Hebei University,Hebei Baoding 071000,China)

机构地区：[1]北京市通州区疾病预防控制中心,北京101100 [2]河北大学,河北保定071000

出　　处：《中国食品卫生杂志》2023年第7期1114-1119,共6页Chinese Journal of Food Hygiene

摘　　要：目的了解通州区2015—2021年食源性疾病监测病例中副溶血弧菌的脉冲场凝胶电泳(PFGE)优势带型、耐药情况和毒力基因携带情况,为副溶血弧菌感染防控和治疗提供参考数据。方法对2015—2021年通州区定点监测医院门诊腹泻病例的粪便样本进行副溶血弧菌分离培养,对其进行血清分型、毒力基因检测、PFGE聚类分析及药敏试验。结果2828份粪便标本中检出副溶血弧菌100株,检出率为3.54%,每年7~9月是检出高峰月份。不同年份副溶血弧菌检出率差异具有统计学意义(χ2=53.94,P<0.001)。100株副溶血弧菌中有一株未携带tlh基因,89.00%的菌株携带致病性毒力基因tdh。副溶血弧菌优势血清型为O3∶K6(66/100),其次是O4∶K8(9/100)。98株菌副溶血弧菌(2株降解)PFGE分型结果显示副溶血性弧菌有39个PFGE带型,命名为V1-V39,条带相似度在79.6%~100%之间,基因分布呈高度多态性,V22和V25是通州区副溶血弧菌的优势带型。菌株对头孢唑林的耐药率最高(32.00%),其次是氨苄西林(14.00%)和多黏菌素E(13.00%),对四环素类、氯霉素类、氨基糖苷类、碳青霉烯类四类药物100%敏感。结论通州区2015—2021年食源性疾病监测病例中检出的副溶血弧菌主要是O3∶K6型tdh+-trh-菌株,对头孢唑林,氨苄西林和多黏菌素E耐药。PFGE图谱主要流行菌株带型相似度在93.1%以上,存在暴发风险,食品安全相关部门需做好副溶血弧菌监测及暴发预警工作,预防食源性疾病暴发。Objective To provide reference data for the prevention,control,and treatment of Vibrio parahaemolyticus infection,the dominant band type of pulsed field gel electrophoresis(PFGE),drug resistance,and virulence gene present in V.parahaemolyticus detected during foodborne disease surveillance cases in Tongzhou district,Beijing,from 2015 to 2021 was investigated.Methods V.parahaemolyticus was isolated and cultured from stool samples of outpatient diarrhea cases in designated monitoring hospitals in Tongzhou district from 2015 to 2021.Serotyping,virulence gene detection,PFGE cluster analysis,and drug sensitivity tests were performed.Results The detection rate of V.parahaemolyticus in 2828 fecal samples was 3.54%,and the peak months of detection were from July to September every year.A statistically significant difference in the detection rate of V.parahaemolyticus in different years(χ^(2)=53.94,P<0.001)was observed.Among the 100 strains of V.parahaemolyticus,one strain did not carry the tlh gene,and 89.00% of the strains carried the pathogenic virulence genes tdh.The dominant serotype of V.parahaemolyticus was O3∶K6(66/100),followed by O4∶K8(9/100).The results of PFGE typing showed that V.parahaemolyticus had 39 band types named V1-V39,the band similarity was between 79.6% and 100%,and the gene distribution was highly polymorphic.V22 and V25 were the dominant band types of V.parahaemolyticus in Tongzhou district.The resistance rate of strains to cefazolin was the highest(32.00%),followed by that to ampicillin(14.00%)and polymyxin E(13.00%),and sensitivity to all the other four types of drugs was 100%.Conclusion V.parahaemolyticus detected in foodborne disease surveillance cases in Tongzhou district from 2015 to 2021 was O3∶K6 type tdh+-trh-strain,which is resistant to cefazolin,ampicillin,and polymyxin E.The similarity of the main epidemic strains in the PFGE pattern is more than 93.1%,and there is an outbreak risk.Food safety departments need to thoroughly monitor V.parahaemolyticus and issue outbreak warnings

关 键 词：副溶血弧菌 毒力基因 血清分型 脉冲场凝胶电泳 耐药 

分 类 号：R155[医药卫生—营养与食品卫生学]