海莲叶提取物抑制非小细胞肺癌A549细胞增殖、侵袭、迁移并促进细胞凋亡  

Extract of Bruguiera sexangula leaves inhibits the proliferation,invasion,migration and promotes the apoptosis of non-small cell lung cancer A549 cells

作　　者：李源 党海舟 刘慧[3] LI Yuan;DANG Haizhou;LIU Hui(Department of Respiratory Medicine,the 3201 Hospital,Hanzhong,723000,Shaanxi,China;Xi'an People's Hospital(Xi'an Fourth Hospital),Xi’an,710000,Shaanxi,China;Chengde Medical University,Chengde,067000,Hebei,China)

机构地区：[1]三二〇一医院呼吸内科,陕西汉中723000 [2]西安市人民医院(西安市第四医院),陕西西安710000 [3]承德医学院,河北承德067000

出　　处：《肿瘤药学》2023年第5期574-581,共8页Anti-Tumor Pharmacy

基　　金：陕西省医学科学研究重点课题计划(2016JM1142)。

摘　　要：目的研究海莲叶提取物对非小细胞肺癌A549细胞增殖、凋亡、侵袭和迁移能力的影响。方法将非小细胞肺癌A549细胞分成Control组(0μg·mL^(-1)海莲叶提取物处理)、BSLE-L组(10μg·mL^(-1)海莲叶提取物处理)、BSLE-M组(20μg·mL^(-1)海莲叶提取物处理)、BSLE-H组(30μg·mL^(-1)海莲叶提取物处理)、BSLE-M+Jagged1-Fc组(20μg·mL^(-1)海莲叶提取物和Notch信号通路激活剂Jagged1-Fc处理)。采用MTT实验检测细胞增殖,平板克隆实验检测细胞克隆形成能力,流式细胞术检测细胞凋亡变化,Transwell小室检测细胞迁移和侵袭能力,Western blotting检测细胞中Bax、Bcl-2、vimentin、E-cadherin、MMP-2、Notch-1、NICD-1和Hes-1蛋白表达变化。结果与Control组比较,BSLE-L、BSLE-M、BSLE-H组细胞存活率和克隆形成数目依次降低,细胞凋亡率依次升高,细胞侵袭数目、迁移数目依次降低,细胞中Bax、E-cadherin蛋白表达水平依次升高,vimentin、MMP-2、Bcl-2、Notch-1、NICD-1和Hes-1蛋白表达水平依次降低,差异均有统计学意义(P<0.05)。与BSLE-M组比较,BSLE-M+Jag‐ged1-Fc组细胞存活率和克隆形成数目显著升高,细胞凋亡率显著降低,细胞迁移和侵袭数目显著升高,细胞中Bax、E-cadherin蛋白表达水平显著降低,Bcl-2、vimentin、MMP-2、Notch-1、NICD-1和Hes-1蛋白表达水平显著升高,差异均有统计学意义(P<0.05)。结论海莲叶提取物可抑制非小细胞肺癌A549细胞增殖、侵袭、迁移、上皮间质转化(EMT),诱导细胞凋亡,其作用机制与降低Notch信号通路激活水平有关。Objective To study the effects of extract of Bruguiera sexangula leaves on the proliferation,apoptosis,inva-sion and migration of non-small cell lung cancer A549 cells.Methods Non-small cell lung cancer A549 cells were divided into Control group(treated with 0μg•mL-1 extract of Bruguiera sexangula leaves),BSLE-L group(treated with 10μg•mL-1 extract of Bruguiera sexangula leaves),BSLE-M group(treated with 20μg•mL-1 extract of Bruguiera sexangula leaves),BSLE-H group(treated with 30μg•mL-1 extract of Bruguiera sexangula leaves),BSLE-M+Jagged1-Fc group(treated with 20μg•mL-1 extract of Bruguiera sexangula leaves and Notch signaling pathway activator Jagged1-Fc).MTT experiment was used to detect the proliferation of cells,and plate cloning experiment to detect the cell clone formation ability,flow cytometry to detect the cell apoptosis changes,Transwell chamber to detect the cell migration and invasion ability,Western blotting to detect the expressions of Bax,Bcl-2,vimentin,E-cadherin,MMP-2,Notch-1,NICD-1 and Hes-1 proteins.Results Com-pared with the Control group,the cell survival rate and the number of clone formation and the number of invasion and migra-tion cells decreased sequentially in the BSLE-L,BSLE-M and BSLE-H groups.The expression levels of Bax and E-cad-herin proteins in cells increased sequentially in the BSLE-L,BSLE-M and BSLE-H groups,and the expression levels of vi-mentin,MMP-2,Bcl-2,Notch-1,NICD-1 and Hes-1 proteins decreased(P<0.05).Compared with the BSLE-M group,the survival rate and the number of cell clone formation,and the number of migration and invasion cells increased significantly in the BSLE-M+Jagged1-Fc group,while the cell apoptosis rate decreased significantly.The expression levels of Bax and E-cadherin proteins in the cells were down-regulated,while the expression levels of Bcl-2,vimentin,MMP-2,Notch-1,NICD-1,Hes-1 proteins were up-regulated(P<0.05).Conclusion Bruguiera sexangula leaves extract inhibited the prolifer-ation,invasion,migration and EMT of non-small cell lun

关 键 词：海莲叶提取物 非小细胞肺癌 迁移 凋亡 Notch信号通路 

分 类 号：R734.2[医药卫生—肿瘤] R285.5[医药卫生—临床医学]