ANLN敲除对肺腺癌细胞生物学功能的影响  

The effect of ANLN knockout on the biological function of lung adenocarcinoma cells

作　　者：朱思明 李标 ZHU Siming;LI Biao(No.1 District of Thoracic Surgery,Second Affiliated Hospital of Hainan Medical University,Haikou,Hainan 570311,China)

机构地区：[1]海南医学院第二附属医院胸外科一区,海南海口570311

出　　处：《现代医药卫生》2023年第23期3961-3965,共5页Journal of Modern Medicine & Health

基　　金：海南省自然科学基金高层次人才项目(SQ2022MSXM1128)。

摘　　要：目的 通过敲除肺腺癌细胞的Anillin肌动蛋白结合蛋白(ANLN)基因,验证其对肺癌细胞增殖、迁移等生物功能及行为学的影响。方法 通过构建ANLN-siRNA质粒,电转染A549肺腺癌细胞,单克隆挑取、鉴定、扩大培养后得到实验组(ANLN-A549组),空载体质粒转染A549细胞得到对照组(Ctrl-A549组)。采用Transwell细胞迁移实验和细胞划痕实验检测2组肺癌细胞的迁移情况;采用CCK-8细胞增殖实验和细胞集落形成实验检测肺癌细胞的克隆增殖能力。结果 Transwell细胞迁移及细胞划痕实验结果表明,ANLN基因敲除后,ANLN-A549组肺癌细胞的迁移率较Ctrl-A549组显著下降,差异有统计学意义(P<0.05);CCK-8细胞增殖实验结果显示,ANLN-A549组较Ctrl-A549组细胞增殖明显减缓,差异有统计学意义(P<0.05);细胞集落形成实验表明,ANLN-A549组较Ctrl-A549组细胞群落数显著减少,差异有统计学意义(P<0.05)。结论 ANLN基因的敲除显著抑制了肺腺癌细胞的增殖、迁移能力。Objective To investigate the effect of Anillin actin-binding protein(ANLN)gene knockout on the proliferation,migration and behavior of lung adenocarcinoma(LUAD)cells.Methods ANLN-siRNA plasmid was constructed and transfected into A549 lung adenocarcinoma cells by electroporation.After monoclonal selection,identification and expansion culture,the experimental group(the ANLN-A549 group)was obtained.The control group(the Ctrl-A549 group)was obtained by transfecting A549 cells with empty vector plasmid.Transwell cell migration assay and cell scratch test were used to detect the migration of lung cancer cells in the two groups,and CCK-8 cell proliferation assay and cell colony formation assay were used to detect the clonal proliferation ability of lung cancer cells.Results Transwell cell migration and cell scratch experiments showed that after ANLN gene knockout,the migration rate of lung cancer cells in the ANLN-A549 group was significantly lower than that in the Ctrl-A549 group,and the difference was statistically significant(P<0.05).The results of CCK-8 cell proliferation assay showed that the proliferation of ANLN-A549 group was significantly slower than that of the Ctrl-A549 group(P<0.05).Cell colony formation assay showed that the number of cell communities in the ANLN-A549 group was significantly lower than that in the Ctrl-A549 group(P<0.05).Conclusion The knockout of ANLN gene significantly inhibits the proliferation and migration of lung adenocarcinoma cells.

关 键 词：Anillin肌动蛋白结合蛋白 肺腺癌 增殖 迁移 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]