PLIN2在氧化型低密度脂蛋白诱导的泡沫细胞脂噬中的作用  

The role of PLIN2 in oxidized low density lipoprotein-induced foam cell lipophagy

作　　者：谭艳美 陈三俊 谭玉林 吴智 蒙国照 谭艳飞 TAN Yanmei;CHEN Sanjun;TAN Yulin;WU Zhi;MENG Guozhao;TAN Yanfei(School of Basic Medicine,Xiangnan University,Chenzhou,Hunan 423000,China;Key Laboratory of Biomedical Microbiology,Xiangnan University,Chenzhou,Hunan 423000,China;Department of Pain,Chenzhou First People′s Hospital,Chenzhou,Hunan 423000,China;Department of Endocrinology Diabetes,Chenzhou First People′s Hospital,Chenzhou,Hunan 423000,China)

机构地区：[1]湘南学院基础医学院,湖南郴州423000 [2]湘南学院生物医药微生物组学重点实验室,湖南郴州423000 [3]郴州市第一人民医院疼痛科,湖南郴州423000 [4]郴州市第一人民医院内分泌糖尿病科,湖南郴州423000

出　　处：《现代医药卫生》2023年第23期3966-3969,3976,共5页Journal of Modern Medicine & Health

基　　金：湖南省教育厅科学研究项目(20C1701);湖南省卫生健康委员会立项课题(D202303067556);湘南学院校级科学研究项目(2020XJ39)。

摘　　要：目的 探讨围脂滴蛋白2(PLIN2)在泡沫细胞脂噬中的作用,为动脉粥样硬化相关疾病的防治提供更多思路。方法 构建氧化型低密度脂蛋白(ox-LDL)诱导的泡沫细胞模型,沉默细胞内PLIN2基因表达,将细胞分为5组:对照组、空质粒组、ox-LDL处理组、siPLIN2组、ox-LDL+siPLIN2组。应用油红O染色法观察细胞内脂质蓄积情况,采用Western blot观察细胞内PLIN2和脂噬相关蛋白LC3和p62表达情况。结果 在ox-LDL诱导的泡沫细胞形成过程中LC3蛋白表达量随着脂质的增加而增加,p62则先减少后增加;在PLIN2沉默的RAW264.7巨噬细胞中LC3表达下调;在PLIN2沉默的泡沫细胞中,p62蛋白表达量明显增加,脂噬减弱。结论 在ox-LDL诱导的泡沫细胞中,敲减PLIN2可下调p62的表达,减弱细胞内脂噬。Objective Foam cell formation was an important part of the occurrence and development of atherosclerosis.To explore the role of perilipin2(PLIN2)in foam cell lipophagy,and to provide more ideas for the prevention and treatment of atherosclerosis-related diseases.Methods The foam cell model induced by oxidized low density lipoprotein(ox-LDL)was constructed,and the expression of PLIN2 gene in the cells was silenced.The cells were divided into five groups:the control group,the empty plasmid group,the ox-LDL treatment group,the siPLIN2 group,the ox-LDL+siPLIN2 group.The oil red O staining was used to observe the lipid accumulation of intracellular,and the Western blot was used to observe the expression of PLIN2 and lipophagy-related proteins LC3 and p62.Results During the formation of foam cells induced by ox-LDL,the expression of LC3 increased with the increase of lipid,while p62 decreased first and then increased.The expression of LC3 was down-regulated in PLIN2-silenced RAW264.7 macrophages.In PLIN2-silenced foam cells,the expression of p62 protein was significantly increased and lipophagy was weakened.Conclusion In ox-LDL-induced foam cells,knockdown of PLIN2 can down-regulate the expression of p62 and attenuate intracellular lipophagy.

关 键 词：脂噬 围脂滴蛋白2 氧化型低密度脂蛋白 泡沫细胞 

分 类 号：R-331[医药卫生]