柱前衍生HPLC-UV法检测蜂蜜中二羟基丙酮的方法研究  

Study on Determination of Dihydroxyacetone in Honey by HPLC with Precolumn Derivatization

作　　者：王圣义 胡‧娟 WANG Sheng-yi;HU Juan(Hubei Yangzijiang Apiculture Co.,Ltd.,Wuhan 430070,China)

机构地区：[1]湖北省扬子江蜂业有限公司,湖北武汉430070

出　　处：《蜜蜂杂志》2023年第12期11-13,共3页Journal of Bee

摘　　要：建立一种柱前衍生HPLC-UV法检测蜂蜜中二羟基丙酮的方法。采用2mL3.0g/L的二硝基苯肼溶液进行二羟基丙酮的柱前衍生,在364nm处进行检测。该方法在2~40mg/kg范围内线性关系良好,提取回收率在102%~110%之间,方法的检测限为0.5mg/kg。该方法检测限低,准确度高,可用于蜂蜜中二羟基丙酮的检测。AHPLC-UV with precolumn derivatization method has been set up to determine the concentration of dihydroxyacetone in honey.The 3.0 g/L 2,4-DNPH was used to derive dihydroxyacetone and tested at the wavelength 364nm,The linear range of method was 2-40 mg/kg.The range of recovery rate was 102%-110%.The detection limit was 0.5 mg/kg.The method is sensitive and accurate,which is suitable for quantitative analysis of dihydroxyacetone in honey.

关 键 词：衍生法 蜂蜜 二羟基丙酮 高压液相色谱法 

分 类 号：S896.1[农业科学—特种经济动物饲养]