槲皮素腹腔注射对大鼠视网膜缺血再灌注损伤的影响及其机制  

Effect and mechanism of intraperitoneal injection of quercetin on retinal ischemia-reperfusion injury in rats

作　　者：于雯 陈肖君 肖正霞 孙艳[1] 夏飞[1] 李娜[1] YU Wen;CHEN Xiaojun;XIAO Zhengxia;SUN Yan;XIA Fei;LI Na(School of Clinical Medicine,The Affiliated Hospital of Weifang Medical University,Weifang 261000,China;不详)

机构地区：[1]潍坊医学院附属医院临床医学院,山东潍坊261000 [2]济南市第二人民医院眼科

出　　处：《山东医药》2023年第29期31-35,共5页Shandong Medical Journal

基　　金：山东省医药卫生科技发展计划项目(202007020634)。

摘　　要：目的观察槲皮素(QU)腹腔注射对大鼠视网膜缺血再灌注损伤(RIRI)的影响并探讨其机制。方法96只成年雄性无眼疾SPF级大鼠采用随机数字法分为对照组6只,RIRI组、阴性对照组及QU组各30只。QU组于建模前连续7 d及建模前10 min腹腔注射QU,RIRI组、阴性对照组分别腹腔注射等量生理盐水及阴性对照溶剂DMSO,对照组不作任何处理。除对照组外,RIRI组、QU组及阴性对照组均采用升高眼压法建立RIRI模型。分别于建模后6、12、24、48、72 h制作大鼠视网膜组织标本,采用HE染色观察大鼠视网膜组织形态变化,计算机图像分析系统测量大鼠视网膜组织内层厚度。采用TUNEL法观察视网膜组织细胞凋亡情况,免疫组织化学染色检测视网膜组织半胱氨酸天冬氨酸蛋白酶3(Caspase-3)表达。结果对照组大鼠视网膜细胞形态及各层分界均正常;RIRI组、阴性对照组大鼠建模6 h后视网膜呈高度水肿,12 h后视网膜神经节细胞数目减少、神经节细胞及内核层细胞排列紊乱,24 h后视网膜水肿较前有所减轻、细胞结构遭到进一步破坏,48 h后可见视网膜水肿基本消失且神经节细胞数、内核层细胞数减少;QU组在各时点视网膜组织结构较RIRI组及阴性对照组更完整,细胞损伤程度更轻。建模后6、12 h视网膜内层厚度RIRI组、阴性对照组>QU组>对照组,建模后24 h视网膜内层厚度QU组>RIRI组、阴性对照组>对照组,建模后48、72h视网膜内层厚度对照组>QU组>RIRI组、阴性对照组(P均<0.05)。对照组视网膜组织偶见凋亡细胞、Caspase-3阳性细胞表达;RIRI组、阴性对照组视网膜组织凋亡细胞数及Caspase-3阳性细胞表达在建模6、12、24h逐渐升高,于建模24h至高峰,并于建模48、72h逐渐降低;QU组各时点视网膜组织凋亡细胞数及Caspase-3阳性细胞表达变化趋势与RIRI组、阴性对照组相同,但均低于同时点RIRI组、阴性对照组(P均<0.05)。结论QU腹腔�Objective To investigate the protective effect of intraperitoneal injection of quercetin(QU)on retinal ischemia-reperfusion injury(RIRI)rats and its mechanism.Methods The random number method was used to divide 96 adult male SPF-grade rats without ocular disease into the control group(6 rats),the RIRI group(30 rats),the negative control group(30 rats),and the QU group(30 rats).At 7 consecutive days before modeling and 10 minutes before model⁃ing,the rats in the QU group underwent the intraperitoneal injection of QU,the rats in the RIRI group underwent the intra⁃peritoneal injection of an equal volume of normal saline,rats in the negative control group were intraperitoneally injected with an equal volume of DMSO,and rats in the control group were not treated.In addition to the control group,the ocular hypertension method was used to establish the RIRI models in the RIRI group,the QU group,and the negative control group.Rat retinal tissue specimens were prepared at 6,12,24,48,and 72 h after modeling.Changes in the histomor⁃phology of the rat retina were observed by hematoxylin-eosin(HE)staining,and the thickness of the inner layer of the rat retinal tissues was measured by a computerized image analysis system.Retinal apoptosis was observed by the TUNEL meth⁃od.The expression of cysteine containing Caspase-3 was detected by immunohistochemistry.Results The retinal cells at each layer were normal in rats of the control group.The retinas of rats in the RIRI group and the negative control group showed a high degree of edema after 6 h of modeling;the number of ganglion cells in the retina was reduced and the ar⁃rangement of ganglion cells and inner nuclear layer cells was disorganized after 12 h of modeling;the edema of the retina was reduced after 24 h of modeling and the structure of the cells was further disrupted;and the edema of the retina was ba⁃sically disappeared and the number of ganglion cells and inner nuclear layer cells was reduced after 48 h of modeling.The QU group had more intact retinal tiss

关 键 词：槲皮素 细胞凋亡 半胱氨酸天冬氨酸蛋白酶3 视网膜缺血再灌注损伤 

分 类 号：R774[医药卫生—眼科]