小鼠中缝背核至丘脑室旁核参与伤害性信息的形态学研究  

Morphological study of nociceptive information transmission from the dorsal raphe nucleus to the paraventricular nucleus of the thalamus in mice

作　　者：冯慧洁 薛美琪 朱慧 张蓬鑫 彭霏 吕培源 阮彩莲[1] 董玉琳 FENG Huijie;XUE Meiqi;ZHU Hui;ZHANG Pengxin;PENG Fei;LYU Peiyuan;RUAN Cailian;DONG Yulin(Department of Anatomy,Medical College,Yan’an University,Yan’an 716000;Department of Anatomy,Histology and Embryology,K.K.Leung Brain Research Centre,Air Force Medical University,Xi’an 710032;The 4 th Battalion,School of Basic Medicine,Air Force Medical University,Xi’an 710032;The 5 th Battalion,School of Basic Medicine,Air Force Medical University,Xi’an 710032,China)

机构地区：[1]延安大学基础医学院人体解剖学教研室,延安716000 [2]空军军医大学基础医学院人体解剖与组织胚胎学教研室暨梁銶琚脑研究中心,西安710032 [3]空军军医大学基础医学院学员四大队,西安710032 [4]空军军医大学基础医学院学员五大队,西安710032

出　　处：《神经解剖学杂志》2023年第5期501-508,共8页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(32271045);陕西省重点研发计划(2023-YBSF-156)。

摘　　要：目的:观察中缝背核(DR)内5-羟色胺(5-HT)能神经元向丘脑室旁核(PVT)内速激肽-1(TAC1)免疫阳性神经元的投射,并观察该通路在伤害性刺激下的激活情况。方法:将逆行示踪剂荧光金(FG)注入小鼠PVT,观察FG逆标神经元在DR内的分布,同时利用免疫组织荧光染色技术观察DR内5-HT能神经元与FG逆标神经元之间的共存情况;继而选用TAC1-ires-Cre小鼠结合RV逆标病毒,在DR观察向PVT内TAC1阳性神经元发出投射的突触前神经元的分布;最后利用TAC1-ires-Cre与Rosa26:CAG-LSL-tdTomato(Ai9)杂交小鼠制备坐骨神经分支损伤(SNI)模型,观察PVT内被激活的TAC1阳性神经元与5-HT能神经终末之间的关系。结果:(1)将FG注入PVT后,在DR的吻、中、尾段均可见FG逆标神经元,其分布以中段为主,吻、尾段数量相对较少,并且在DR内观察到5-HT和FG的双标神经元。(2)向TAC1-ires-Cre小鼠的PVT内注射RV逆行跨突触三联病毒后,在DR内也可见RV标记的突触前神经元,主要分布于DR中段。(3)SNI模型下TAC1-Cre::Ai9杂交小鼠PVT内部分TAC1神经元被激活,且同时与5-HT能神经终末形成密切接触。结论:DR内5-HT能神经元向PVT内TAC1阳性神经元发出投射,形成DR 5-HT+-PVT TAC1+神经通路,该通路可以被伤害性信息激活,提示其可能在伤害性信息传递/调控过程中发挥一定的作用。Objective:To observe the projection of 5-HTergic neurons in the dorsal raphe nucleus(DR)to the TAC1 positive neurons of paraventricular nucleus of the thalamus(PVT)and the activation of this specific neuronal pathway under chronic pain condition.Methods:The retrograde tracer fluorogold(FG)was injected into the PVT to observe the distribution of retrogradely labeled neurons and the coexistence between 5-HTergic neurons and retrogradely labeled neurons in the DR.Then,the presynaptic neurons of the TAC1 positive neurons of the PVT in the DR were studied using RV viral system combined TAC1-ires-Cre mice.Finally,the activation of TAC1 positive neurons received projection from the DR was observed by using TAC1-ires-Cre::Ai9 mice with a spared nerve injury(SNI)model.Results:(1)After FG was injected into the PVT,FG retrogradely labeled neurons were distributed in the rostral to caudal part of the DR,with the dominance in the middle part.The number of FG retrogradely labeled neurons in the rostral and caudal part was relatively small.And the double-labeled neurons of 5-HT/FG could be found in the DR.(2)RV-labeled presynaptic neurons were also observed in the DR after RV viral system injected into the PVT of TAC1-ires-Cre mice,and mostly distributed in the middle of DR.(3)The close appositions could be observed in TAC1/FOS double labeled neurons and the 5-HTergic axonal terminals under chronic pain condition.Conclusion:The DR 5-HT+-PVT TAC1+neural pathway is formed by projecting of 5-HTergic neurons in the DR to the TAC1 positive neurons in the PVT,which can be activated by nociceptive stimulation,suggesting that it may play a role in the processing of nociception transmission/regulation.

关 键 词：中缝背核 丘脑室旁核 5-羟色胺 速激肽-1 伤害性信息 小鼠 

分 类 号：R-332[医药卫生]