基于F_(1)群体的玉米株高与穗位高的全基因组关联分析  

Genome-wide Association Study of Maize Plant Height and Ear Height Based on F_(1) Population

作　　者：梁航宇 叶凡 董远[1,2] 李婷 段迎新 刘斌斌 李周帅 徐淑兔 张兴华[1,2] 薛吉全 LIANG Hangyu;YE Fan;DONG Yuan;LI Ting;DUAN Yingxin;LIU Binbin;LI Zhoushuai;XU Shutu;ZHANG Xinghua;XUE Jiquan(College of Agronomy,Northwest A&F University/Key Laboratory of Biology and Genetic Improvement of Maize in Arid Area of Northwest Region,Ministry of Agriculture and Rural Affairs,Yangling Shaanxi 712100,China;Maize Engineering Technology Research Centre of Shaanxi Province,Yangling Shaanxi 712100,China)

机构地区：[1]西北农林科技大学农学院/农业农村部西北旱区玉米生物学与遗传育种重点实验室,陕西杨凌712100 [2]陕西省玉米工程技术研究中心,陕西杨凌712100

出　　处：《西北农业学报》2023年第12期1879-1887,共9页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：现代农业产业技术体系专项(玉米)(CARS-02)。

摘　　要：株高、穗位高是影响玉米耐密性的重要株型性状,明确玉米株高、穗位高的遗传基础有利于株型改良。基于NCⅡ遗传交配设计,以陕A群和陕B群选育的85个自交系组配的246份F1群体为材料,进行株型表型评价,同时结合55 951个高质量SNPs标记,对株高、穗位高以及穗位系数进行全基因组关联分析。结果表明:株高、穗位高、穗位系数的遗传力分别为77.68%、77.04%、73.78%,且三者之间存在显著正相关。同时,通过全基因组关联分析检测到7、5、4个SNP与株高、穗位高和穗位系数显著相关,解释1.47%~25.27%表型变异。通过候选基因功能注释,共筛选到10个候选基因,涉及植物的生长发育、生物合成、响应非生物胁迫等过程,针对3个共定位区间和热点区间锚定 plt1、 atp2、 ZC3H46、 emp21等为候选基因。可见,通过株型表型鉴定及相关基因的挖掘,可为陕A群和陕B群两个玉米群体的株型改良提供理论基础。Plant and ear height are key plant architecture traits that affect density tolerance of maize.Understanding the genetic basis of plant and ear height can aid in enhancing the plant architecture,particularly in maize hybrids.This study evaluated plant and ear height in 246 F_(1) hybrids constructed from 85 inbred lines selected from Shaan A and Shaan B group,using the NCII genetic mating design.This study also conducted a genome-wide association study using 55951 high-quality SNPs markers to identify the genetic basis of plant height(PH),ear height(EH),and EH/PH.The heritability of plant height,ear height and ear ratio was high,ranging from 77.68%,77.04%and 73.78%,respectively,and there was a high significant positive correlation among them.The genome-wide association study identified 7,5 and 4 SNPs associated with plant height,ear height and EH/PH,respectively.These SNPs explained 1.47%-25.27%of phenotypic variation.Functional annotation of candidate genes revealed 10 potential candidate genes involved in plant growth and development,biosynthesis process and response to abiotic stress process.This study also identified three co-localization intervals and hot spot intervals where genes such as plt1,atp2,ZC3H46 and emp21 were anchored.These results provide a theoretical basis for enhancing plant architecture in the Shaan A and Shaan B maize populations.

关 键 词：玉米 杂交种 株高 穗位高 全基因组关联分析 

分 类 号：S513[农业科学—作物学]