Development and Validation of UHPLC-MS/MS Method for Quantifying of Agarotriose:An Application for Pharmacokinetic,Tissue Distribution,and Excretion Studies in Rats  

作　　者：YUE Jiali CHENG Wei WEI Shutong LIU Guilin ZHOU Meichen LV Zhihua YU Mingming 

机构地区：[1]School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China [2]Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266003,China [3]Key Laboratory of Glycoscience&Glycotechnology of Shandong Province,Qingdao 266003,China [4]Key Laboratory of Marine Drugs,Ministry of Education of China,Qingdao 266003,China

出　　处：《Journal of Ocean University of China》2023年第6期1683-1691,共9页中国海洋大学学报（英文版）

基　　金：funded by the Fundamental Research Funds for the Central Universities(Nos.201912008,201964019);the Natural Science Foundation of Shandong Province(No.ZR2019BC025).

摘　　要：A sensitive,rapid,and robust ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)method was established for the first time to quantify agarotriose(A3)in rat plasma,tissues,urine,and feces.A3 and stachyose(internal standard)were separated by a BEH amide column at 65℃under the mobile phase of 10 mmol L^(-1)ammonium ace-tate-acetonitrile(42:58,v/v)with 350µLmin-1.The acquisition of transitions was carried out in multiple reaction monitoring(MRM)pattern operating with positive ionization at m/z 509.16>329.15 for A3 and m/z 689.15>527.11 for stachyose.The linearity ranges of A3 were 10 to 5000nmolL^(-1)for plasma,20 to 10000nmolL^(-1)for tissues,and 40 to 20000nmolL^(-1)for urine and feces.The accuracy and precision ranged from 90.9%to 111.6%and 0.7%to 10.1%,respectively.The stability was between 86.1%and 102.5%.The extraction recovery was consistent and reproducible.The matrix effect ranged from 1.5%to 11.4%.The pharmacokinetic,tissue dis-tribution,and excretion studies were successfully conducted with the validated method.Results showed that A3 could be absorbed by rats,and the absolute bioavailability was 6.7%.Furthermore,it was rapidly distributed in rat tissues and mainly eliminated via feces excretion(67.0%)after oral administration.For intravenous bolus,85.5%was recovered,and renal excretion was the primary path-way(77.6%)for cumulative recovery.

关 键 词：agarotriose UHPLC-MS/MS PHARMACOKINETIC tissue distribution EXCRETION 

分 类 号：R965[医药卫生—药理学]