血卟啉衍生物在3种肺癌细胞系中的细胞摄取和定位  

UPTAKE AND LOCALIZATION OF HEMATOPORPHYRIN DERIVATIVES IN THREE LUNG CANCER CELL LINES

作　　者：马亿江 丁晓倩[1] 隋爱华[2] 杨晓晖 曹艺巍[1] 林存智[1] MA Yijiang;DING Xiaoqian;SUI Aihua;YANG Xiaohui;CAO Yiwei;LIN Cunzhi(Department of Respiratory and Critical Care Medicine,The Affiliated Hospital of Qingdao University,Qingdao 266555,China)

机构地区：[1]青岛大学附属医院呼吸与危重症医学科,山东青岛266555 [2]青岛大学附属医院医学研究中心,山东青岛266555

出　　处：《青岛大学学报（医学版）》2023年第5期703-708,共6页Journal of Qingdao University(Medical Sciences)

基　　金：北京科创医学发展基金会项目(KC2021-JX-0186-64)。

摘　　要：目的以支气管上皮BEAS-2B细胞系为对照,比较不同肺癌细胞系(肺腺癌A549、肺鳞癌H520、肺小细胞癌H446细胞)之间血卟啉衍生物(HPD)的细胞摄取和定位差异。方法用不同浓度HPD测得的荧光值绘制HPD荧光值的标准曲线。将4种细胞分别与HPD孵育不同时间后,用多功能酶标仪检测荧光强度。进一步用流式细胞仪量化细胞摄取,比较4种细胞HPD摄取差异。用激光扫描共聚焦显微镜观察比较细胞内HPD摄取和分布情况。结果4种细胞系摄取HPD随着时间增加而增加,但HPD的累积速率有所差异。5 mg/L的HPD孵育细胞24、48 h,4种细胞内平均荧光强度差异有统计学意义(F=199.00、71.15,P<0.001),其中A549细胞内平均荧光强度显著高于H446、BEAS-2B、H520细胞,H446和BEAS-2B细胞内平均荧光强度显著高于H520细胞(P<0.05)。15 mg/L的HPD孵育细胞48 h,4种细胞内呈现出强度不等的红色荧光信号,均呈点状分布于细胞质内。结论不同类型肺癌细胞系摄取HPD存在差异,其中肺腺癌A549细胞摄取HPD的能力最强,支气管上皮BEAS-2B细胞与肺小细胞癌H446细胞次之,肺鳞癌H520细胞摄取能力最弱;HPD细胞内定位均位于细胞质内,呈点状分布。Objective To compare the differences in cellular uptake and localization of hematoporphyrin derivatives(HPDs)between different lung cancer cell lines(lung adenocarcinoma A549,lung squamous cell carcinoma H520,and lung small cell carcinoma H446)using the bronchial epithelial cell line BEAS-2B as control.Methods The standard curve of HPD fluorescence values was plotted using values measured at different concentrations of HPDs.The four cell lines were incubated with HPDs for different time periods and then measured for fluorescence intensity using a multifunctional microplate meter.Flow cytometry was used to quantify the cell uptake and compare the differences in HPD uptake between the four cell lines.The uptake and distribution of HPDs in the cells were observed and compared using a laser scanning confocal microscope.Results The uptake of HPDs in the four cell lines increased over time,with some differences in the accumulation rate of HPDs.After cells were incubated with 5 mg/L HPD for 24 h and 48 h,the mean fluorescence intensities of the four cell lines were significantly different(F=199.00,71.15,P<0.001),with the mean fluorescence intensity significantly higher in A549 cells than in H446,BEAS-2B,and H520 cells,and the mean fluorescence intensity significantly higher in H446 and BEAS-2B cells than in H520 cells(P<0.05).The four cell lines showed varying intensities of red fluorescent signals distributed in a dot pattern in the cytoplasm when the cells were incubated with 15 mg/L HPDs for 48 h.Conclusion There are differences in HPD uptake between different lung cancer cell lines,with the highest uptake in A549 cells,followed by BEAS-2B and H446 cells,and the lowest uptake in H520 cells.HPDs are located in the cytoplasm of all the cell lines,showing a dotted distribution pattern.

关 键 词：肺肿瘤 血卟啉衍生物 光化学疗法 细胞摄取 细胞内定位 

分 类 号：R734.2[医药卫生—肿瘤] R349.21[医药卫生—临床医学]