Bna-miR43介导甘蓝型油菜响应干旱胁迫  

Bna-miR43 Mediates the Response of Drought Tolerance in Brassica napus

作　　者：张盈川 吴晓明玉 陶保龙 陈丽[1,2] 鲁海琴 赵伦 文静 易斌[1] 涂金星[1] 傅廷栋[1] 沈金雄[1] Yingchuan Zhang;Xiaomingyu Wu;Baolong Tao;Li Chen;Haiqin Lu;Lun Zhao;Jing Wen;Bin Yi;Jinxing Tu;Tingdong Fu;Jinxiong Shen(National Engineering Research Center of Rapeseed/National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China;School of Advanced Agriculture and Bioengineering,Yangtze Normal University,Chongqing 408100,China)

机构地区：[1]华中农业大学作物遗传改良全国重点实验室/国家油菜工程技术研究中心,武汉430070 [2]长江师范学院现代农业与生物工程学院,重庆408100

出　　处：《植物学报》2023年第5期701-711,共11页Chinese Bulletin of Botany

基　　金：湖北省重点研发计划(No.2022ABA001)。

摘　　要：miRNA通过调控靶基因的表达参与植物生长发育和响应逆境胁迫等多个方面。对前期鉴定到的1个miRNA——Bna-miR43进行功能研究,通过构建过表达载体探讨了Bna-miR43在甘蓝型油菜(Brassica napus)响应干旱胁迫中的功能。降解组测序预测到Bna-miR43的4个靶基因均属于F-box蛋白家族。在模拟干旱条件下,甘蓝型油菜J572根系Bna-miR43的表达量逐渐下降;靶基因则呈现与Bna-miR43相反的表达模式,且随着干旱处理时间的增加,靶基因的表达量逐渐上升。转基因实验表明,在干旱胁迫下,油菜Bna-miR43过表达株系表现为对干旱极度敏感,转基因株系种子在干旱条件下发芽率显著下降,植株脱水严重,体内积累了更多的丙二醛(MDA)和过氧化氢(H2O2)。干旱处理后, Bna-miR43过表达株系中编码超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和乙醇酸氧化酶(GOX)的3个基因下调表达。上述结果表明,Bna-miR43通过调控靶基因的表达调节甘蓝型油菜渗透物质积累和细胞活性氧(ROS)稳态,在调控植物耐旱性中发挥重要作用。miRNAs are involved in plant growth and response to stress by regulating the expression of target genes.We conducted a functional study on a single miRNA identified by our group,Bna-miR43.The function of Bna-miR43 under drought stress was investigated by constructing a Bna-miR43 overexpression vector.Degradation group sequencing has predicted that the four target genes of Bna-miR43 belonged to the F-box protein family.Under simulated drought conditions,the expression of Bna-miR43 in J572 roots decreased gradually,while the expression pattern of target genes was shown to be opposite to that of Bna-miR43.With the increaseing time of drought treatment,the expression of target genes increased gradually.Expression pattern analysis showed that Bna-miR43 could respond to drought stress by negatively regulating the expression of target genes.Transgenic experiment showed that under drought stress,the Bna-miR43 overexpressed lines were extremely sensitive to drought.It was shown that the germination rate of the overexpressed lines decreased significantly,the plant dehydration was serious,and more MDA and H2O2 were accumulated in the body.After drought treatment,three coding superoxide dismutase(SOD),catalase(CAT)and glycolate oxidase(GOX)were identified in the Bna-miR43 overexpressed lines,and their expressions were down-regulated in the Bna-miR43 overexpressed lines.The results showed that Bna-miR43 plays a key role in regulating plant drought tolerance by regulating the osmotic accumulation and ROS homeostasis in Brassica napus.

关 键 词：甘蓝型油菜 Bna-miR43 F-BOX 干旱胁迫 

分 类 号：S565.4[农业科学—作物学]