Mangiferin alleviates renal infammatory injury in spontaneously hypertensive rats by inhibiting MCP-1/CCR2 signaling pathway  

作　　者：Xiaoqin Hu Wei Zhao Jiagang Deng Zhengcai Du Xuewen Zeng Bei Zhou Erwei Hao 

机构地区：[1]College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530001,China [2]Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica,Nanning 530001,China [3]Jinhua Advanced Research Institute,Jinhua 321000,China

出　　处：《Chinese Herbal Medicines》2023年第4期556-563,共8页中草药（英文版）

基　　金：supported by Natural Science Foundation of Guangxi Province (No. 2013GXNSFAA019114);Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica Project (No. 12-071-08)。

摘　　要：Objective: Hypertension is a low-grade infammation state of the disease and was easily complicated by kidneys’ infammatory response. Mangiferin(MGF), a pharmacologically active compound in various plants including Mangifera indica, has a strong anti-infammatory activity. However, the effects of MGF on renal infammatory injury in spontaneously hypertensive rats(SHRs) remain unclear. The purpose of this study was to investigate the protective effects and mechanisms of MGF on renal infammatory injury in SHRs.Methods: MGF was used in SHRs at the doses of 10, 20, 40 mg/kg/d for 8 weeks consecutively. The blood and urine were collected for assessment of renal function. Renal tissues were collected for histological,immunohistochemistry, ELISA, Western blot and real time reverse transcription PCR(RT-PCR) analysis.Results: The results showed that the levels of interleukin 6(IL-6), tumor necrosis factor-a(TNF-a), monocyte chemoattractant protein-1(MCP-1) and recombinant chemokine C-C-Motif receptor 2(CCR2) were increased in SHRs, meanwhile, the level of IL-10 was decreased in SHR. Treatment of MGF inhibited the expression of IL-6, TNF-a, MCP-1 and CCR2, and promoted the expression of IL-10. Furthermore, the content of blood urea nitrogen(BUN) and serum uric acid(SUA) was significantly increased in the model group, and treatment of MGF had no obvious effects on these parameters at all dose levels.Conclusion: Our study proved that the kidneys of SHRs had significant infammatory injury, and MGF had the protective effects on renal infammatory injury in SHRs;The protective mechanism may be mediated partly by the MCP-1/CCR2 signaling pathway. Thus, it is a potential new drug for the treatment of hypertension.

关 键 词：HYPERTENSION MANGIFERIN MCP-1/CCR2 renal inflammatory injury spontaneously hypertensive rat 

分 类 号：R285.5[医药卫生—中药学]