经尾动脉入路原发性肝癌大鼠TACE模型的构建  

The establishment of TACE model via transcaudal arterial access in experimental rats with hepatocellular carcinoma

作　　者：洪笑阳 刘键鑫 郭钊雄 黄文薮 梁伟 郭永建 王荣坤 林立腾 朱康顺 HONG Xiaoyang;LIU Jianxin;GUO Zhaoxiong;HUANG Wensou;LIANG Wei;GUO Yongjian;WANG Rongkun;LIN Liteng;ZHU Kangshun(Department of Interventional Radiology,Minimally-Invasive and Interventional Cancer Center,Affiliated Second Hospital of Guangzhou Medical University,Guangzhou,Guangdong Province 510000,China)

机构地区：[1]广州医科大学附属第二医院微创介入科,广东广州510000

出　　处：《介入放射学杂志》2023年第11期1096-1100,共5页Journal of Interventional Radiology

基　　金：吴阶平医学基金会(320.6750.2020-10-63);广东省普通高校青年创新人才项目(2020KQNCX057);广州医科大学附属第二医院临床科研项目(2021-LCYJ-01)。

摘　　要：目的建立经尾动脉途径肝癌大鼠经导管肝动脉化疗栓塞术(TACE)模型,并探讨其可行性、有效性和安全性。方法 0.01%二乙基亚硝胺(DEN)溶液连续诱导大鼠12周建立肝癌模型,12周后通过MRI明确肿瘤大小。当最大肿瘤长径>5 mm时,在DSA引导下经尾动脉插管,并将微导管置于肝固有动脉进行TACE,治疗后复查CT了解瘤内碘油沉积。观察大鼠术后1周生存率及相关并发症情况。检测术前及术后3、7 d大鼠ALT、AST、TBil及CRE水平。术后7 d行肝、肺、双肾及脾脏组织病理学检查。结果 TACE模型成功建立,尾动脉置管用时(6.9±1.4) min。造影显示大鼠肝内肿瘤染色明显,术后CT显示瘤内碘油沉积密实,组织病理学结果显示肿瘤大面积坏死,坏死区周缘Caspase3、HIF-1 alpha表达水平明显升高。肺、肾、脾脏大体标本未发现明显异位栓塞。所有大鼠行为无异常。术后3 d大鼠ALT、AST、TBil较术前明显上升(P<0.05),术后7 d明显下降,但仍高于术前(P<0.05),手术前后CRE水平无明显差异(P>0.05)。结论 经尾动脉入路可以安全、有效地完成对大鼠原发性肝癌的TACE治疗。Objective To establish a TACE model via transcaudal arterial access in experimental rats with diethylnitrosamine-induced(DEN-induced)hepatocellular carcinoma(HCC),and to evaluate its feasibility,effectiveness and safety.Methods The experimental rats were treated with 0.01%diethylnitrosamine(DEN)solution for 12 weeks to induce spontaneous HCC.After 12 weeks,the tumor size was determined by magnetic resonance imaging(MRI).When the maximum long diameter of the tumor was>5 mm,DSA-guided catheterization with a microcatheter via transcaudal arterial access into the proper hepatic artery was performed,which was followed by TACE.CT reexamination was adopted to check the lipiodol deposition within the tumor.The survival rate and associated complications at one week after surgery were recorded.The levels of aspartate aminotransferase(ALT),alanine aminotransferase(AST),total bilirubin(TBil),and creatinine(CRE)were determined before TACE as well as at 3 days and 7 days after TACE.The histopathological examinations of the liver,lung,both kidneys and spleen were performed 7 days after TACE.Results The TACE model was successfully established,and an average time of(6.9±1.4)minutes was spent for transcaudal artery catheterization.Angiography showed that significant staining of the liver tumor in rats was observed.Postoperative CT scan revealed that dense lipiodol deposition was found within the tumor.The histopathological examination results showed that the tumor showed large areas of necrosis,and the expression levels of Caspase3 and HIF-1 alpha in the peripheral margin of the necrotic area were significantly increased.No obvious ectopic embolism was found in the gross specimens of the lung,kidney,and spleen.No abnormal behaviors were observed in all of the rats.The postoperative 3-day levels of ALT,AST and TBil in rats were significantly increased when compared with their preoperative levels(P<0.05),which decreased significantly at 7 days after TACE,but still higher than their preoperative levels(P<0.05).There was no statistic

关 键 词：介入放射学 肝细胞癌 大鼠 治疗性化疗栓塞 尾动脉 

分 类 号：R735.7[医药卫生—肿瘤]