MTA联合CGF用于大鼠磨牙直接盖髓效果的研究  

Study on the effect of MTA combined with CGF on direct pulp capping of rat molars

作　　者：麦天倩 李春年[1] 许明明 MAI Tianqian;LI Chunnian;XU Mingming(School and Hospital of Stomatology,Hebei Medical University&Hebei Key Laboratory of Stomatology,The Oral Disease Clinical Medicine Research Institution of Hebei,Shijiazhuang 050017)

机构地区：[1]河北医科大学口腔医学院·口腔医院,河北省口腔医学重点实验室/河北省口腔疾病临床医学研究中心,石家庄050017

出　　处：《现代口腔医学杂志》2023年第6期391-395,共5页Journal of Modern Stomatology

基　　金：河北省医学科学课题重点研究计划(20210047);河北省2022年政府资助临床优秀人才项目(361029);河北省省级科技计划资助(20377761D)。

摘　　要：目的探讨无机三氧化物聚合物(mineral trioxide aggregate,MTA)联合浓缩生长因子(concentrate growth factors,CGF)用于大鼠磨牙直接盖髓术的效果。方法将16只SD大鼠处死并分离下颌第一磨牙,将磨牙自牙颈部截断,保留牙冠及冠髓,根据盖髓材料的不同将牙冠随机分为:空白对照组、MTA组、CGF组、MTA+CGF组,盖髓后将样本植入裸鼠背部皮下,6周后取出样本进行HE染色、Masson染色及免疫组化染色,并对染色结果进行分析。结果(1)盖髓6周后,MTA+CGF组较MTA组、CGF组诱导形成的牙髓-牙本质复合体结构更为典型;(2)MTA+CGF盖髓与单独应用MTA、CGF盖髓对钙化桥的形成及牙髓组织的影响并无明显差异(P>0.05);(3)牙本质涎磷蛋白(dentin salivary phosphoprotein,DSPP)在MTA+CGF组表达最强,各组之间差异具有统计学意义(P<0.05)。结论MTA联合CGF用于大鼠磨牙直接盖髓比单独应用MTA、CGF盖髓效果更佳,在活髓保存术中具有较大的潜能及应用价值。Objective To investigate the effect of Mineral Trioxide Aggregate(MTA)combined with Concentrate Growth Factors(CGF)for direct pulp capping in rat molars.Methods Sixteen SD rats were sacrificed and mandibular first molars were separated.The molars were cut off from the cervical part of the teeth,and the crown and pulp were preserved.After the pulp was covered,the samples were implanted subcutaneously into the back of nude mice.After 6 weeks,the samples were removed for HE staining,Masson staining and immunohistochemical staining.Results①After 6 weeks of pulp capping,the pulp-dentin complex structure of MTA+CGF group was more typical than that of MTA group and CGF group.②There was no significant difference in the formation of calcified bridge and the effect on the pulp tissue between MTA+CGF and MTA or CGF alone(P>0.05);Dentin Salivary Phosphoprotein(DSPP)expression was the strongest in MTA+CGF group,and the differences among groups were statistically significant(P<0.05).Conclusion MTA combined with CGF is more effective than either MTA or CGF alone in pulp capping.MTA combined with CGF has great potential and application value in vital pulp therapy.

关 键 词：无机三氧化物聚合物 浓缩生长因子 直接盖髓 活髓保存术 牙本质涎磷蛋白 

分 类 号：R781.3[医药卫生—口腔医学]