基于生物信息学分析前列腺癌关键miRNAs与差异基因的研究  

Bioinformatics-based analysis of key miRNA

作　　者：林澍坤 门波[2] 张宸铭[1] 马思成 华众 张芳[1] Lin Shukun;Men Bo;Zhang Chenming;Ma Sicheng;Hua Zhong;Zhang Fang(Second Clinical Medical College,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,Henan,China;Henan Provincial Hospital of Traditional Chinese Medicine,Zhengzhou 450002,Henan,China)

机构地区：[1]河南中医药大学第二临床医学院,河南郑州450046 [2]河南省中医院,河南郑州450002

出　　处：《中国男科学杂志》2023年第5期111-119,共9页Chinese Journal of Andrology

摘　　要：目的通过生物信息学分析影响前列腺癌进展的关键miRNA及其靶基因。方法从GEO数据库获取前列腺癌miRNA数据集GSE36802及前列腺癌mRNA数据集GSE46602、GSE69223,通过GEO2R筛选差异表达的miRNAs,利用miRWalk、miRPathDB对显著差异表达的miRNA进行靶基因预测并取交集。随后将筛选出前列腺癌相关差异表达基因与预测靶基因取交集最终得到差异表达基因。运用DAVID数据库对差异表达基因进行GO和KEGG富集分析,使用String数据库构建蛋白-蛋白互作网络,应用Cytoscape软件与CytoHubba插件筛选枢纽基因并通过GEPIA2数据库进行验证。结果共获得4个在前列腺癌组织中上调的miRNAs,分别是miR-145-3p、miR-205-5p、miR-31-5p、miR-455-5p。最终共得出差异表达基因153个,其主要富集在RNA聚合酶II启动子转录的调节、信号转导、细胞黏附等生物过程及代谢途径通路、癌症通路、钙信号通路等通路中。通过蛋白互作网络得到10个备选枢纽基因,利用GEPIA2对枢纽基因进行表达差异验证,最终得到4个枢纽基因:IGF1、PPARGC1A、FGFR2、DPT。结论本研究筛选出4个差异表达miRNAs和4个枢纽基因,为后续临床治疗和基础研究提供参考。Objective To discover key miRNAs and their target genes affecting the progression of prostate cancer by bioinformatics analysis.MethodssThe prostate cancer miRNA dataset CSE36802 and prostate cancer mRNA datasets GSE46602 and GSE69223 were obtained from the GEO database,and the differentially expressed miRNAs were screened by GEO2R,and the target genes were predicted using miRWalk and miRPathDB and the intersection sets were taken.Subsequently,prostate cancer-related differentially expressed genes were analyzed and intersected with predicted target genes to finally obtain differentially expressed genes.The differentially expressed genes were analyzed by GO and KEGG enrichment using DAVID database,protein-protein interaction network was constructed using String database,and the pivotal genes were screened by Cytoscape software and CytoHubba plugin and validated by GEPIA2 database.Results A total of four miRNAs were obtained that were upregulated in prostate cancer tissues,namely miR-145-3p,miR-205-5p,miR-31-5p,and miR-455-5p.153 differentially expressed genes were finally obtained,which were mainly enriched in biological processes such as regulation of RNA polymerase II promoter regulation,signal transduction,cell adhesion,and in signal transduction such as metabolic pathway,cancer pathway,calcium signaling pathway.Ten alternative pivotal genes were obtained by protein interaction network,and the differential expression of pivotal genes was verified by using GEPIA2,and finally four pivotal genes were obtained including:ICFI,PPARGCIA,FCFR2,and DPT.Conclusion Four differentilly expressed miRNAs and four pivotal genes were discovered to provide the potential target for subsequent clinical treatment and basic research.

关 键 词：前列腺肿瘤 生物信息学 差异表达基因 微RNAS 

分 类 号：R737.25[医药卫生—肿瘤] R73-051[医药卫生—临床医学]