离子交换色谱法测定饲料中含硫氨基酸含量的不确定度评定  

Uncertainty evaluation of determination of sulfur amino acids in feed by ion exchange chromatography

作　　者：汪园 马家麟 王环[1] 谭亮[1] 李玉林[1] WANG Yuan;MA Jia-lin;WANG Huan;TAN Liang;LI Yu-lin

机构地区：[1]中国科学院西北高原生物研究所分析测试中心,青海西宁810001

出　　处：《饲料研究》2023年第23期126-130,共5页Feed Research

基　　金：兰州区域中心自主部署课题(项目编号:lz2025ff01)。

摘　　要：试验采用离子交换色谱法测定饲料中含硫氨基酸的含量,对含硫氨基酸检测结果的不确定度进行计算和评定。试验建立了数学模型以对整个试验过程的不确定度进行来源分析,计算合成不确定度和扩展不确定度。结果显示,牧草粗饲料中胱氨酸的含量为0.39 g/100 g,扩展不确定度为0.02 g/100 g (k=2);蛋氨酸的含量为0.21 g/100 g,扩展不确定度为0.02 g/100 g (k=2),方法回收率和标准溶液配制的不确定度贡献较大,是测量误差的主要来源。研究表明,试验建立的不确定度评定方法可为饲料中含硫氨基酸含量的质量控制提供参考。The study used ion exchange chromatography to determine the content of sulfur amino acids in feed,and calculated and evaluated the uncertainty of sulfur amino acids.A mathematical model was established to analyze the sources of uncertainty,calculate the composite uncertainty and the expanded uncertainty.The results showed that the content of cystine in feed was 0.39 g/100 g,with an extended uncertainty of 0.02 g/100 g(k=2),while the content of methionine was 0.21 g/100 g,with an expanded uncertainty of 0.02 g/100 g(k=2).The primary source of measurement error is due to the uncertainty of recovery rate and standard solution preparation.The study indicates that the uncertainty evaluation method established in the study can provide valuable guidance for the quality control of sulfur amino acids in feed.

关 键 词：离子交换色谱法 饲料 含硫氨基酸 不确定度 

分 类 号：S816.17[农业科学—饲料科学]