A recently evolved BAHD acetyltransferase,responsible for bitter soyasaponin A production,is indispensable for soybean seed germination  

作　　者：Jia Yuan Liya Ma Yan Wang Xindan Xu Rui Zhang Chengyuan Wang Wenxiang Meng Zhixi Tian Yihua Zhou Guodong Wang 

机构地区：[1]State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,The Innovative Academy of Seed Design,the Chinese Academy of Sciences,Beijing 100101,China [2]College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing 100039,China [3]State Key Laboratory of Molecular Developmental Biology,the Chinese Academy of Sciences,Beijing 100190,China [4]The Center for Microbes,Development and Health,Institute of Pasteur of Shanghai,the Chinese Academy of Sciences,Shanghai 200031,China [5]State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,The Innovative Academy of Seed Design,the Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Journal of Integrative Plant Biology》2023年第11期2490-2504,共15页植物学报（英文版）

基　　金：financially supported by National Key Research and Development Projects (2021YFF1001200 and 2018YFA0900603);“Priority Research Program”of the Chinese Academy of Science (Grant No.ZDRW-ZS-2019-2);the State Key Laboratory of Plant Genomics of China (SKLPG2016A-13)to GW。

摘　　要：Soyasaponins are major small molecules that accumulate in soybean(Glycine max)seeds.Among them,type-A soyasaponins,fully acetylated at the terminal sugar of their C22 sugar chain,are responsible for the bitter taste of soybean-derived foods.However,the molecular basis for the acetylation of type-A soyasaponins remains unclear.Here,we identify and characterize Gm SSAc T1,encoding a BADH-type soyasaponin acetyltransferase that catalyzes three or four consecutive acetylations on type-A soyasaponins in vitro and in planta.Phylogenetic analysis and biochemical assays suggest that Gm SSAc T1 likely evolved from acyltransferases present in leguminous plants involved in isoflavonoid acylation.Loss-of-function mutants of Gm SSAc T1 exhibited impaired seed germination,which attribute to the excessive accumulation of null-acetylated type-A soyasaponins.We conclude that Gm SSAc T1 not only functions as a detoxification gene for high accumulation of type-A soyasaponins in soybean seeds but is also a promising target for breeding new soybean varieties with lower bitter soyasaponin content.

关 键 词：BAHD acyltransferase seed germination SOYASAPONIN soybean 

分 类 号：S565.1[农业科学—作物学]