牦牛CAPS基因克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of Calcyphosine Gene(CAPS)in Yak

作　　者：李卿正 张玉洁 黄鑫蕊 卢丹 曹萍 马志杰[1] 李瑞哲 LI Qingzheng;ZHANG Yujie;HUANG Xinrui;LU Dan;CAO Ping;MA Zhijie;LI Ruizhe(Academy of Animal and Veterinary Science,Qinghai University,Qinghai Key Laboratory of Protection and Innovative Utilization,Qinghai Yak Engineering Technology Research Center,Xining 810016,China;College of Ecological and Environmental Engineering,Qinghai University,Xining 810016,China;Kunlun College,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学畜牧兽医科学院,青海省高原家畜遗传资源保护与创新利用重点实验室,青海省牦牛工程技术研究中心,青海西宁810016 [2]青海大学生态环境工程学院,青海西宁810016 [3]青海大学昆仑学院,青海西宁810016

出　　处：《青海畜牧兽医杂志》2023年第4期1-5,38,共6页Chinese Qinghai Journal of Animal and Veterinary Sciences

基　　金：青海省“昆仑英才·高端创新创业人才”计划;青海省科技厅项目(2023-NK-135)。

摘　　要：钙磷蛋白(calcyphosine,CAPS)是一种钙结合蛋白,对维护生物体内钙磷平衡起着重要作用。本研究克隆了牦牛CAPS基因的CDS区序列,分析了其核苷酸序列,并进行了生物信息学分析。结果表明,牦牛CAPS基因CDS区全长570 bp,编码189个氨基酸;编码蛋白的分子式为C_(905)H_(1434)N_(266)O_(297)S_(8),分子量为21049.43 Da,理论等电点为4.69,脂溶指数为75.29,不稳定指数为37.52,是疏水性稳定蛋白;系统进化分析表明,牦牛CAPS基因CDS区与普通牛和瘤牛的基本一致,与水牛、绵羊和猪的序列一致性较高。本研究为深入研究牦牛CAPS的生理功能提供了参考资料。As a calcium-binding protein,calcyphosine(CAPS)plays an important role in maintaining the balance of calcium and phosphorus in vivo.In this study,the CDS region of yak CAPS gene was cloned,its nucleo-tide sequence was analyzed,and bioinformatics analysis was carried out.Results showed that the CDS region of CAPS gene was 570 bp long and encoded 189 amino acids.The formula,molecular weight,theoretical pI,aliphat-ic index,instability index of the encoded protein was C_(905)H_(1434)N_(266)O_(297)S_(8),21049.43 Da,4.69,75.29 and 37.52,respectively,which suggested it was a stable fat-soluble protein.Phylogenetic analysis showed that the CDS region of CAPS gene in yak had the highest similarity with that of common cattle and Zebu,with higher simi-larity with buffalo,sheep and pig.This study provided reference datafor further study on the physiological function of CAPS in yak.

关 键 词：牦牛 钙磷基因 基因克隆 生物信息学分析 系统进化 

分 类 号：S823.85[农业科学—畜牧学]