基于微卫星标记探究5个虹鳟和金鳟养殖群体的遗传多样性  

Genetic Diversity Evaluation of 5 Cultured Rainbow Trout Populations Based on Microsatellite Markers

作　　者：杨顺文 杨濯羽 苏子郡 焦文龙 卡伟 史小宁 YANG Shunwen;YANG Zhuoyu;SU Zijun;JIAO Wenlong;KA Wei;SHI Xiaoning(Gansu Fisheries Research Institute,Lanzhou 730000,China;Gansu Fishery Resources and Environment in the Upper Reaches of the Yellow River Observation and Research Station,Lanzhou 730030,China)

机构地区：[1]甘肃省水产研究所,甘肃兰州730000 [2]甘肃省黄河上游渔业资源环境野外科学观测研究站,甘肃兰州730030

出　　处：《青海畜牧兽医杂志》2023年第4期47-52,63,共7页Chinese Qinghai Journal of Animal and Veterinary Sciences

基　　金：甘肃省中央引导地方科技发展专项“甘肃省黄河上游渔业资源环境野外科学观测研究站建设项目”(YDZX20206200001155)资助;甘肃省自然科学基金——甘肃金鳟伪雄鱼诱导研究(23JRRA1333)资助。

摘　　要：本研究采用8对微卫星DNA标记,对道氏虹鳟、挪威虹鳟、波兰金鳟、丹麦金鳟和甘肃金鳟5个养殖鳟鱼群体进行遗传多样性分析。结果表明,8个微卫星座位共检测到111个等位基因,平均He为13.875,PIC在0.838~0.933之间。对5个鳟鱼群体进行遗传多样性评价,平均He在0.599~0.832之间,平均Ho在0.605~0.788之间,平均PIC在0.532~0.808之间。通过研究认为,5个鳟鱼养殖群体整体上仍有较高的遗传多样性,其中2个金鳟群体遗传多样性明显低于虹鳟群体,在进行选育时应注意保护金鳟群体的遗传多样性不再降低。In this study,8 microsatellite markers were selected to analyze the genetic diversity and population structure of 5 Oncorhynchus mykiss populations,including Donaldsons rainbow trout,Rainbow trout from Norway,Golden trout from Polish,Golden trout from Denmark and Golden trout from Gansu.The results showed that a total of 111 alleles were detected in the 8 microsatellite loci,the average He was 13.875,and the PIC was between 0.838 and 0.933.The genetic diversity of five trout populations was evaluated,and the average He range was 0.599~0.832,average Ho range was 0.605~0.788,and average PIC range was 0.532~0.808.According to the study,the genetic diversity of the five trout populations was still high.The genetic diversity of two popula-tions of golden trout was significantly lower than that of rainbow trout population,therefore the genetic diversity of golden trout population should not be reduced when local selection or artificial breeding was carried out.

关 键 词：遗传多样性 微卫星 虹鳟 金鳟 

分 类 号：S917.4[农业科学—水产科学]