粘红酵母产β-胡萝卜素的发酵条件优化及包埋工艺研究  

Optimization of Fermentation Conditions for β-Carotene Yield by Using Rhodotorula Mucilaginosa and Embedding Process of β-Carotene

作　　者：朱闻凯 李安军 何宏魁 谢雨朦 余子骏 宋歆妍 秦黎明 梅漫莉 曹润洁 曾化伟[1] 马金同 ZHU Wenkai;LI Anjun;HE Hongkui;XIE Yumeng;YU Zijun;SONG Xinyan;QIN Liming;MEI Manli;ZENG Huawei;MA Jintong(Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation,Department of Bioengineering,College of Life Science,Huaibei Normal University,Huaibei 235000,China;Anhui Gujing Gongjiu Co.,Ltd,Bozhou 236800,China;Anhui Reswell Technology Co.,Ltd,Bozhou 236800,China)

机构地区：[1]淮北师范大学生命科学学院安徽省污染物敏感材料与环境重点实验室,淮北235000 [2]安徽古井贡酒股份有限公司,亳州236800 [3]安徽瑞思威尔科技有限公司,亳州236800

出　　处：《中央民族大学学报（自然科学版）》2023年第4期52-59,共8页Journal of Minzu University of China(Natural Sciences Edition)

基　　金：安徽省科技厅重大科技专项(202103a06020004)。

摘　　要：β-胡萝卜素是一种重要的动物饲料添加剂,微生物液体发酵合成天然β-胡萝卜素具有极大应用前景。本文通过粘红酵母发酵产β-胡萝卜素的单因素优化和正交优化实验,获得最佳的培养基为柠檬酸浓度为40 g/L,牛肉膏浓度为20 g/L,番茄汁的浓度为6 mg/L,初始pH值为6。5,β-胡萝卜素产量达到788.88ug/L。本实验进一步采用β-环糊精进行包埋β-胡萝卜素,存放10 d后保留率为57.80%,相比于未包埋的β-胡萝卜素的保留率提高了28.61%。本研究为微生物产β-胡萝卜素产量提高及其稳定性的提升提供了依据。β-carotene is an important animal feed additive.The synthesis of natural β-carotene by microbial liquid fermentation has great application prospects.The highest β-carotene yield(788.88 ug/L) of Rhodotorula glutinis was obtained by single factor optimization and orthogonal optimization experiments,and the optimal mediumas as follows:citric acid concentration of 40g/L,beef extract concentration of 20g/L,tomato juice concentration of 6 mg/L,initial pH value at 6.5.In this experiment,β-cyclodextrin was further used to encapsulate β-carotene,and the retention rate was 57.80 % after 10 days of storage,which was 28.57 % higher than that of unencapsulated β-carotene.This study provides a basis for the improvement of microbial β-carotene production and its stability.

关 键 词：粘红酵母 Β-胡萝卜素 培养基配方优化 正交 包埋 

分 类 号：Q591[生物学—生物化学]