基于“质-量”双标的当归质量分析方法研究  

Research on quality analysis method of Angelica sinensis based on“quality-quantity”double standard

作　　者：李晓晨 李天娇[1,2,3] 包永睿 王帅[1,2,3] 孟宪生 LI Xiao-chen;LI Tian-jiao;BAO Yong-rui;WANG Shuai;MENG Xian-sheng(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;Liaoning Multi-dimensional Analysis of Traditional Chinese Medicine Technical Innovation Center,Dalian 116600,China;Liaoning Modern Traditional Chinese Medicine Research and Engineering Laboratory,Dalian 116600,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]辽宁省中药多维分析专业创新技术中心,辽宁大连116600 [3]辽宁省现代中药研究工程实验室,辽宁大连116600

出　　处：《中草药》2023年第22期7313-7318,共6页Chinese Traditional and Herbal Drugs

基　　金：辽宁省教育厅创新团队项目(LT2017015)。

摘　　要：目的建立以对照药材为基准物质定性和不完全依赖对照品定量的当归Angelica sinensis药材“质-量”双标控制方法。方法采用HPLC技术建立当归对照药材的特征图谱,将其与供试药材图谱进行比对来辨别药材的真伪,即“质”;采用内标物质对特征峰化学成分进行相对定量来区分药材的优劣,即“量”。结果建立的当归药材“质-量”分析方法符合方法学考察要求,各供试药材与对照药材的相似度均大于0.98;规定了当归药材特征峰化学成分相对含量下限。结论该方法不依赖多种对照品,能清晰、快速地判断药材的真伪优劣,弥补目前当归药材质量控制的不足,完善中药材质量控制体系,降低中药材(饮片)检测成本,减轻中药企业经济负担,以期推动中医药事业可持续发展。Objective To establish a“quality-quantity”double standard control method for Angelica sinensis,which is qualitative based on the reference traditional Chinese medicinal materials(TCMM)as standard substances and quantitative without relying on multiple reference substances.Methods HPLC technology was used to establish the characteristic chromatogram of the TCMM of A.sinensis,which was compared with the chromatogram of the tested TCMM to identify the authenticity of the medicinal materials,that is,“quality”.The internal standard substance was used to relatively quantify the chemical composition of characteristic peaks to distinguish the quality of medicinal materials,that is,“quantity”.Results The established“quality-quantity”analysis method of A.sinensis met the requirements of methodology investigation,and the similarity between the tested TCMM and the reference TCMM was greater than 0.98.The lower limit of relative content of chemical components of characteristic peaks of A.sinensis was defined.Conclusion This method can judge the authenticity of medicinal materials clearly and quickly without many reference substances,make up for the shortage of quality control of A.sinensis at present,improve the quality control system of TCMM,reduce the testing cost of TCMM(decoction pieces),reduce the economic burden of traditional Chinese medicine enterprises,and promote the sustainable development of traditional Chinese medicine.

关 键 词：当归 对照药材 质量控制 特征图谱 绿原酸 阿魏酸 洋川芎内酯Ⅰ 藁本内酯 

分 类 号：R286[医药卫生—中药学]