Six new coumarins from the roots of Toddalia asiatica and their anti-inflammatory activities  

作　　者：HE Haoxuan LI Niping FAN Yunqi HUANG Qian SONG Jianguo LV Lixia LIU Fen WANG Lei WANG Qi GU Jihong 

机构地区：[1]Science and Technology Innovation Center,Guangzhou University of Chinese Medicine,Guangzhou 510405,China [2]State Key Laboratory of Bioactive Molecules and Druggability Assessment,Jinan University,Guangzhou 510632,China

出　　处：《Chinese Journal of Natural Medicines》2023年第11期852-858,共7页中国天然药物（英文版）

基　　金：supported financially by the National Natural Science Foundation of China(No.82104014 and 82003605);the Key Laboratory Project of Colleges and Universities in Guangdong Province(No.2019KSYS005);the Guangdong Province Science and Technology Plan International Cooperation Project(No.2020A0505100052)。

摘　　要：We reported the discovery of six novel coumarins, toddasirins A–F ( 1 – 6 ), each endowed with modified isoprenyl or geranyl side chains, derived from the roots of Toddalia asiatica. Comprehensive structural elucidation was achieved through multispectroscopic analyses, single-crystal X-ray diffraction experiments, and advanced quantum mechanical electronic circular dichroism (ECD) calculations. Furthermore, the anti-inflammatory activity of these compounds was assessed. Notably, compounds 1 – 3 and 6 demonstrated notable inhibitory effects on nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW 264.7 cells, with 50% inhibitory concentration (IC50) values of 3.22, 4.78, 8.90, and 4.31 μmol·L^(−1), respectively.

关 键 词：Toddalia asiatica COUMARINS Anti-inflammatory effects RAW 264.7 cells 

分 类 号：R284[医药卫生—中药学]