血府逐瘀胶囊联合顺铂对小鼠Lewis肺癌移植瘤血管新生及VEGFA、VEGFR2表达的影响  被引量:3

Effect of Capsule for removing blood stagnation in blood organ combined with cisplatin on angiogenesis and expression of VEGF and VEGFR2 in Lewis lung cancer xenografts in mice

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作  者:王一铮[1] 高冬[1] 王鹤[1] 苏孙益 秦崇涛[1] 王虹 林凡[1] WANG Yizheng;GAO Dong;WANG He;SU Sunyi;QIN Chongtao;WANG Hong;LIN Fan(College of Integrated Traditional and Western Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian,China;Fujian Provincial Hospital,Fuzhou 350001,Fujian,China)

机构地区:[1]福建中医药大学中西医结合学院,福建福州350122 [2]福建省立医院,福建福州350001

出  处:《现代中西医结合杂志》2023年第20期2775-2780,共6页Modern Journal of Integrated Traditional Chinese and Western Medicine

基  金:国家自然科学基金项目(82204969,82074191);福建省自然科学基金项目(2020J01717,2021J01892)。

摘  要:目的探讨血府逐瘀胶囊联合顺铂对小鼠Lewis肺癌移植瘤血管新生及血管内皮生长因子A(VEGFA)、血管内皮细胞生长因子受体2(VEGFR2)表达的影响。方法从75只C57BL/6小鼠中随机取15只作为正常组,其余小鼠均进行Lewis肺癌荷瘤造模。将造模成功的60只小鼠随机分为模型组、顺铂组、血府逐瘀胶囊组、血府逐瘀胶囊联合顺铂组,每组15只。血府逐瘀胶囊组给予血府逐瘀胶囊0.36 g/kg灌胃,模型组给予生理盐水灌胃,均2次/d;顺铂组给予2 mg/kg的顺铂腹腔注射,2 d 1次;血府逐瘀胶囊联合顺铂组给予0.36 g/kg血府逐瘀胶囊灌胃(2次/d)和2 mg/kg的顺铂腹腔注射(2 d 1次);正常组不给予任何干预。各组连续干预15 d后,统计存活率,摘取瘤组织并称量瘤重,计算抑瘤率,免疫组化法检测肿瘤组织中微血管数量,ELISA法测定血浆VEGFA、碱性成纤维细胞生长因子(bFGF)水平,免疫印迹法检测肿瘤组织中VEGFA、VEGFR2蛋白表达情况。结果顺铂组和血府逐瘀胶囊联合顺铂组小鼠存活率明显高于模型组和血府逐瘀胶囊组(P均<0.05);血府逐瘀胶囊联合顺铂组瘤重明显低于其他组(P均<0.05),抑瘤率明显高于顺铂组和血府逐瘀胶囊组(P均<0.05),肿瘤组织中微血管数明显少于模型组和血府逐瘀胶囊组(P均<0.05);顺铂组瘤重明显低于模型组和血府逐瘀胶囊组(P均<0.05),抑瘤率明显高于血府逐瘀胶囊组(P<0.05),肿瘤组织中微血管数明显少于其他组(P均<0.05)。顺铂组血浆VEGFA、bFGF水平和肿瘤组织中VEGFA、VEGFR2蛋白相对表达量均明显低于模型组(P均<0.05);血府逐瘀胶囊联合顺铂组血浆bFGF水平和肿瘤组织中VEGFR2蛋白相对表达量均明显低于模型组(P均<0.05)。结论血府逐瘀胶囊联合顺铂对Lewis肺癌荷瘤小鼠具有显著的抑瘤及抑制肿瘤血管新生的作用,其机制可能与VEGFA/VEGFR2信号通路相关。Objective It is to investigate the effect of Capsule for removing blood stagnation in blood organ(CRBSBO)combined with cisplatin on angiogenesis and the expression of vascular endothelial growth factor A(VEGFA)and vascular endothelial growth factor receptor 2(VEGFR2)in Lewis lung cancer xenografts in mice.Methods Fifteen mice from 75 C57BL/6 mice were randomly selected as the normal group,and the rest of the mice were used to establish Lewis lung cancer bearing mice models.The 60 successfully modeled mince were randomly divided into model group,cisplatin group,CRBSBO group,and CRBSBO+cisplatin group,with 15 mice in each group.The CRBSBO group was given 0.36 g/kg of CRBSBO by gavage,while the model group was given physiological saline by gavage,both twice daily;the cisplatin group was given 2 mg/kg of cisplatin intraperitoneally,once every two days;the CRBSBO+cisplatin group was given 0.36 g/kg of CRBSBO by gavage(twice daily)and 2 mg/kg of cisplatin intraperitoneally(once every two days);the normal group was not given any medication.After 15 days of continuous administration,the survival rate was calculated,the tumor tissues were extracted and weighed to calculate the tumor inhibition rate,the microvessel density of tumor tissue in each group;was detected by immunohistochemistry,the plasma levels of VEGFA and basic fibroblast growth factor(bFGF)were determined by ELISA,and the expressions of VEGFA and VEGFR2 proteins in tumor tissues were detected by immunoblotting.Results The survival rates of mice in the cisplatin group and CRBSBO+cisplatin group were significantly higher than those in the model group and CRBSBO group(all P<0.05);the tumor weight in the CRBSBO+cisplatin group was significantly lower than that in the other groups(P<0.05),the tumor inhibition rate was significantly higher than that in the cisplatin group and CRBSBO group(both P<0.05),and the number of microvessels in the tumor tissues was significantly lower than that in the model group and CRBSBO group(both P<0.05);the tumor weight in the cispla

关 键 词:血府逐瘀胶囊 血管新生 LEWIS肺癌 VEGFA VEGFR2 

分 类 号:R-332[医药卫生]

 

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