MicroRNA-766-5p靶向SCAI对宫颈癌细胞增殖、周期和凋亡的作用  

Effects of MicroRNA-766-5p on cervical cancer cell proliferation,cell cycle and apoptosis by targeting SCAI

作　　者：刘倩男 刘伟伟[2] 毕利红 孙红[2] 张凯[2] Liu Qiannan;Liu Weiwei;Bi Lihong;Sun Hong;Zhang Kai(The Second Ward of Gynecology,Weifang People's Hospital,Weifang 262500,China;The Second Ward of Gynecology,Weifang Yidu Central Hospital,Weifang 262500,China)

机构地区：[1]潍坊市人民医院妇科二病区,山东潍坊262500 [2]潍坊市益都中心医院妇科二病区,山东潍坊262500

出　　处：《实用妇科内分泌电子杂志》2023年第24期1-3,共3页Electronic Journal of Practical Gynecological Endocrinology

基　　金：2020年度山东省医药卫生科技发展计划项目(编号202005011368)。

摘　　要：目的研究MicroRNA-766-5p靶向肿瘤细胞侵袭抑制因子(SCAI)对宫颈癌细胞增殖、周期和凋亡的作用。方法选取人宫颈癌MCF-7细胞模型;CCK8检测细胞增殖;流式细胞术分析细胞周期;Western blot检测细胞周期和凋亡相关蛋白表达。结果MicroRNA-766-5p靶向SCAI高效抑制人宫颈癌MCF-7细胞增殖,48 h处理的IC50为(49.33±7.02)nmol/L。低、高药物浓度实验组减少B细胞特性莫洛尼鼠白血病病毒插入位点1(BMI-1)和细胞周期素D1(CyclinD1)、细胞周期蛋白D1(CyclinD1),增加细胞周期素B1(CyclinB1)和P21,G2/M期周期阻滞,促进促凋亡蛋白[Bcl-2相关X蛋白(Bax)、裂解型多聚ADP-核糖聚合酶(c-PARP)]和抑制凋亡蛋白Bcl-2表达。结论MicroRNA-766-5p靶向SCAI能抑制宫颈癌细胞增殖,促进细胞周期阻滞和凋亡。Objective To investigate the effects of MicroRNA-766-5p targeting suppressor of cancer cell invasion(SCAI)on the proliferation,cycle and apoptosis of cervical cancer cells.Methods Human cervical cancer MCF-7 cell model was selected.Cell proliferation was detected by CCK8.The cell cycle was analyzed by flow cytometry.Western blot was used to detect the expression of cell cycle and apoptosis-related proteins.Results MicroRNA-766-5p effectively inhibited the proliferation of human cervical cancer MCF-7 cells by targeting SCAI,and the IC50 of 48 h treatment was(49.33±7.02)nmol/L.The B-cell characteristics of Moloni mouse leukemia virus insertion site 1(BMI-1),cyclin D1(CyclinD1)and cyclin D1(CyclinD1)were decreased in low and high drug concentration groups,while cyclin B1(CyclinB1)and P21 were increased,and G2/M phase cycle arrest was observed.Promote the expression of pro-apoptotic protein[Bcl-2 associated X protein(Bax),lytic poly ADP-ribose polymerase(c-PARP)]and anti-apoptotic protein Bcl-2.Conclusion MicroRNA-766-5p targeting SCAI can inhibit the proliferation of cervical cancer cells and promote cell cycle arrest and apoptosis.

关 键 词：MicroRNA-766-5p 肿瘤细胞侵袭抑制因子 宫颈癌 细胞增殖 细胞周期 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]