鼻咽癌DLC1基因的表达研究  

Study of DLC1 gene expression in nasopharyngeal carcinoma

作　　者：彭宏[1] 赵彤[2] 姚开泰[2] 

机构地区：[1]第一军医大学南方医院耳鼻咽喉科,广州510515 [2]第一军医大学肿瘤研究所

出　　处：《中华耳鼻咽喉科杂志》2002年第6期454-457,I006,共5页Chinese Journal of Otorhinolaryngology

基　　金：广东省自然科学基金 ( 0 10 60 4);全军医药卫生科研基金( 30 0 0 0 0M2 0 1)

摘　　要：目的　探讨DLC1(deletedinlungcancer 1)基因在鼻咽癌发生中的作用。方法　应用逆转录 -聚合酶链反应 (reversetranscription polymerasechainreaction ,RT PCR)方法检测 4种鼻咽癌细胞株、3 2例鼻咽癌组织和 16例正常鼻咽部组织DLC1基因的表达 ,对发现的异常转录进行DNA测序分析。结果　 4种鼻咽癌细胞株均不表达DLC1基因 ,鼻咽癌组织DLC1基因表达明显低于正常鼻咽部组织 (P <0 0 0 1) ;局部T3、T4期鼻咽癌DLC1基因表达明显低于T1、T2期 (P =0 0 1) ;然而 ,DLC1基因表达与鼻咽癌患者的年龄、性别、颈部淋巴结转移 ,全身远处转移、TNM临床分期和血清EB病毒VCA IgA抗体滴度均无相关性。 3 2例鼻咽癌组织中有 7例发生DLC1基因第 8外显子的异常剪接 ,而 16例正常鼻咽部组织中仅 1例在表达正常DLC1转录的同时伴有该异常转录的表达。结论　DLC1基因的表达异常与鼻咽癌的发生可能密切相关 ,其低表达可能与鼻咽癌的局部侵袭力有关。DLC1基因第Objective To elucidate the role of DLC1 gene in nasopharyngeal carcinoma (NPC). Methods The expression of DLC1 was studied by means of RT-PCR combined with sequencing in 32 cases non-keratinizing carcinoma, 16 cases of histologically normal nasopharyngeal epithelia, and 4 NPC cell lines (CNE1?CNE2 and HNE1?HNE2). Results The expression of functional transcript of DLC1 was undetected in all NPC cell lines, and it was significantly reduced or undetected in NPC biopsies in comparison with normal nasopharyngeal mucosae (P<0.001). The expression of DLC1 was significantly reduced in T3, T4 stages of NPC biopsies, compared with that in T1, T2 stages (P=0.01). However, there was no relationship between the expression of DLC1 and other clinical features in NPC patients, such as age, sex, lymph node metastasis, distant metastasis, TNM clinical stages and the serum titer of EBV (VCA/IgA) antibody (P>0.05). An aberrant transcript of DLC1, which lacked exon 8, was found in 7 cases of NPC and only in 1 case of normal nasopharyngeal mucosae. Conclusion DLC1 gene may play a critical role in the tumorgenesis of NPC. The down-expression of DLC1 may be associated with the local invasion of NPC. The aberrant isoform may be related with the dysfunction of DLC1 gene.

关 键 词：鼻咽癌 DLC1基因 基因表达 

分 类 号：R739.63[医药卫生—肿瘤]