生物信息学分析人类病毒互作广谱蛋白的特征  

Characterization of Broad-spectrum Protein Interactions Between Human and Viral Proteins Through Bioinformatics Analysis

作　　者：庹超皓 彭友松 孟祥贤[1] 朱兆中 TUO Chaohao;PENG Yousong;MENG Xiangxian;ZHU Zhaozhong(College of Biology,Hunan University,Changsha 410000,China;School of Public Health,University of South China,Hengyang 421001,China)

机构地区：[1]湖南大学生物学院,长沙410000 [2]南华大学公共卫生学院,衡阳421001

出　　处：《病毒学报》2023年第6期1528-1540,共13页Chinese Journal of Virology

基　　金：国家重点研发计划(项目号:2022YFC2303802),题目:新型流感病毒动态演化和跨种传播的微观驱动因素及其机制研究;国家自然科学基金(项目号:32170651),题目:发展计算方法预测人类病毒受体。

摘　　要：了解病毒与人类蛋白的相互作用对于理解病毒感染宿主机制非常重要,已有研究主要集中在病毒如何进入宿主细胞、复制、扩散和致病等方向上,对病毒与人类蛋白的相互作用模式缺乏系统研究。本研究中我们构建了迄今为止最全面的病毒与人类之间的蛋白相互作用网络,涵盖来自218种病毒的1674种蛋白与来自人类的13724种蛋白的108832对蛋白相互作用;在此基础上鉴定出109个至少与12种病毒家族存在蛋白相互作用的人类蛋白,定义为人类的病毒互作广谱蛋白(简称广谱蛋白);从结构、功能、蛋白互作网络以及组织表达量等四个方面系统地分析了广谱蛋白的特征,发现广谱蛋白相较于非广谱蛋白以及其它人类蛋白具有更密集的转角结构、更多的结构域、更高的网络中心度和组织表达量,表明它们可能在病毒感染宿主过程中起着重要作用。本研究有助于加深我们对于病毒感染人类模式的理解,同时也对进一步探究病毒与疾病之间的关联有一定的帮助。Understanding the interaction between viruses and human proteins is crucial for comprehending the mechanism of viral infection in the host.Although it has been studied focusing on how viruses enter host cells,replicate,disseminate,and induce diseases,there is a lack of systematic research on the interaction patterns between viruses and human proteins.In this study,we constructed the most comprehensive protein-protein interaction network between viruses and humans to date,which covers 1,674 viral proteins from 218 virus species and 10,832 protein-protein interactions with 13,724 human proteins.Based on this network,we identified 109 human proteins that interact with at least 12 virus families and defined them as broad-spectrum virus-human interaction proteins(broad-spectrum proteins).We systematically analyzed the structure,function,protein interaction network,and tissue expression characteristics of these broad-spectrum proteins.We found that the broad-spectrum proteins have a more compactα-helical structure,a larger number of structural domains,higher network centrality and tissue expression levels compared to other human proteins.These results suggest that they may play crucial roles in the process of viral infection of the host.This study helps to deepen our understanding of the viral infection mechanisms in humans and may also aid in further exploring the relationship between viruses and diseases.

关 键 词：病毒人类蛋白互作 生物信息学 计算生物学 广谱特征 病毒互作蛋白 

分 类 号：R373.9[医药卫生—病原生物学]