微小RNA-138调控Wnt通路对体外人脑胶质瘤细胞生物学行为的影响  

Effect of microRNA-138 regulation of Wnt pathway on the biological behavior of human glioma cells in vitro

作　　者：吴俊波 杨杰 肖锋 李金亮 WU Jun-bo;YANG Jie;XIAO Feng;LI Jin-liang(Department of Neurosurgery,Pingxiang Second People’s Hospital,Jiangxi Pingxiang 337000,China;Department of Neurosurgery,the Third People’s Hospital of Pingxiang City,Jiangxi Pingxiang 337099,China)

机构地区：[1]江西省萍乡市第二人民医院神经外科,江西萍乡337000 [2]江西省萍乡市第三人民医院神经外科,江西萍乡337099

出　　处：《解剖学报》2023年第6期682-688,共7页Acta Anatomica Sinica

基　　金：江西省卫生健康委科技计划项目(202212091)。

摘　　要：目的 探讨微小RNA(miR)-138调控Wnt信号通路对脑胶质瘤细胞生物学行为的影响。方法 选取脑胶质瘤细胞系U-87MG、U251,将其随机分为空白组、miR对照组(miR-NC)、miR-138模拟物组(miR-138 mimics)和miR-138抑制剂组(miR-138 inhibitor)。采用Real-time PCR检测各组细胞中miR-138表达;采用MTT、流式细胞术、Transwell实验及划痕实验分别对各组细胞的增殖、凋亡、侵袭以及迁移能力进行检测,采用Western blotting检测各组细胞Wnt通路相关蛋白表达。结果 MiR-138 mimics组miR-138表达水平较其余3组高,miR-138 inhibitor组低于空白组和miR-NC组(P<0.05);与空白组相比,miR-138 mimics组细胞增殖率较低,miR-138 inhibitor组较高,且成时间依赖性(P<0.05);MiR-138 mimics组细胞凋亡率高于空白组、miR-NC组和miR-138 inhibitor组,而miR-138 inhibitor组细胞凋亡率均低于其余组(P<0.05);MiR-138 mimics组侵袭细胞数低于空白组、miR-NC组和miR-138 inhibitor组,而miR-138 inhibitor组均高于其余3组(P<0.05);MiR-138 mimics组细胞迁移率低于空白组、miR-NC组和miR-138 inhibitor组,而miR-138 inhibitor组均高于其余3组(P<0.05);MiR-138 mimics组Wnt3a、Wnt1、糖原合成激酶3β(GSK-3β)和β-连环蛋白(β-catenin)蛋白表达低于空白组、miR-NC组和miR-138 inhibitor组;而miR-138 inhibitor组均高于其余3组(P<0.05)。结论 MiR-138过表达可有效抑制脑胶质瘤细胞增殖、侵袭和迁移并促进其凋亡,可能是通过抑制Wnt信号通路而实现。Objective To investigate the effect of microRNA(miR)-138 regulation of Wnt signaling pathway on the biological behavior of human glioma cells in vitro. Methods Glioma cell lines U-87MG and U251 were selected and randomly divided into blank group, miR-NC group, miR-138 mimics group and miR-138 inhibitor group. Real-time PCR was used to detect the miR-138 expression in each group;MTT, flow cytometry, Transwell assay and scratch assay were used to detect proliferation, apoptosis, invasion and migration ability of each group respectively, and Western blotting was used to detect Wnt pathway-related protein expression in each group. Results The miR-138 expression level was higher in the miR-138 mimics group compared with the remaining 3 groups, and that in the miR-138 inhibitor group was lower than that in the blank group and the miR-NC group(P<0.05);Compared with the blank group, the cell proliferation rate was lower in the miR-138 mimics group and higher in the miR-138 inhibitor group, and was time-dependent(P<0.05);The apoptosis rate in the miR-138 mimics group was higher than that in the blank group, miR-NC group, and miR-138 inhibitor group, while the apoptosis rate in the miR-138 inhibitor group was lower than that in the rest other groups(P<0.05);The number of cell-invading cells in the miR-138 mimics group was lower than that in the blank group, miR-NC group, and miR-138 inhibitor group, while all miR-138 inhibitor group were higher than the remaining three groups(P<0.05);The cell migration rate of miR-138 mimics group was lower than that of blank group, miR-NC group and miR-138 inhibitor group, while all miR-138 inhibitor group were higher than the remaining three groups(P<0.05);Wnt3a, Wnt1, glycogen synthase kinase 3β(GSK-3β) and β-catenin protein expression in the miR-138 mimics group was lower than that in the blank group, miR-NC group, and miR-138 inhibitor group;While miR-138 inhibitor groups were higher than the remaining three groups(P<0.05). Conclusion MiR-138 overexpression effectively inhibite the

关 键 词：微小RNA-138 脑胶质瘤 WNT信号通路 生物学行为 免疫印迹法 

分 类 号：R739.41[医药卫生—肿瘤]