用于核酸提取样本容积最优化的等频率运动控制  

Equal-frequency motion control for volume optimization of nucleic acid extraction samples

作　　者：蒋新 姚佳[2,3] 李东书 王天一 李金泽 李莹雪 周连群 JIANG Xin;YAO Jia;LI Dongshu;WANG Tianyi;LI Jinze;LI Yingxue;ZHOU Lianqun(Xuzhou Medical University,Xuzhou 221000,China;Key Laboratory of Biomedical Laboratory Technology,Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215163,China;Suzhou Guoke Xingan Medical Technology Company,Suzhou 215163,China)

机构地区：[1]徐州医科大学,江苏徐州221000 [2]中国科学院苏州生物医学工程技术研究所中国科学院生物医学检验技术重点实验室,江苏苏州215163 [3]苏州国科芯感医疗科技有限公司,江苏苏州215163

出　　处：《中国测试》2023年第11期157-165,共9页China Measurement & Test

基　　金：国家自然科学基金青年基金资助项目(61901469);江苏省重点研究开发项目(BE2020768);中国科学院科研仪器设备研制项目(YJKYYQ20200046)。

摘　　要：为实现核酸提取振荡运动下样本容积最优化,将等频率运动控制算法应用于核酸提取过程中,通过提高核酸提取运动过程的平稳性,使振荡运动下核酸样本容积得到有效提升。基于搭建的STM32高精度控制平台,通过等频率离散化的方式控制步进电机使用Sigmoid曲线实现加减速过程,实现步进电机的平滑、精确控制。提出的等频率离散加减速运动控制算法可解决变速过程中速度变化不平稳问题。对比等时间采样的实验表明,相同实验参数下,采用等频率离散的方式比等时间离散能更快达到目标频率。在精度对比上,采用等频率离散的方式在定位精度上比等时间离散法提高39.47%。在核酸提取应用中,等频率离散法比等时间离散法可有效提高核酸样本容积20.62μL。与传统Sigmoid函数模型实现S型曲线加减速控制算法相比,等频率离散法可提升运动定位精度使抽提过程更加顺滑,有效提高了核酸提取振荡运动下的样本容积。In order to optimize the sample volume of nucleic acid extraction under oscillating motion,the equal frequency motion control algorithm is applied to the nucleic acid extraction process.By improving the stability of the nucleic acid extraction process,the sample volume of nucleic acid under oscillating motion is effectively improved.Based on the STM32 high precision control platform,the step motor was controlled by equal frequency discretization.The Sigmoid curve was used to realize the acceleration and deceleration process,and the smooth and accurate control of the step motor was realized.The proposed constant frequency discrete acceleration and deceleration motion control algorithm solved the problem of unstable speed change in the process of variable speed.Compared with equal-time sampling,the experimental results show that under the same experimental parameters,the method of equal-frequency discretization can achieve the target frequency faster than that of equal-time discretization.Compared with the equal time discretization method,the equal frequency discretization method improves the positioning accuracy by 39.47%.In the application of nucleic acid extraction,the constant frequency dispersion method can effectively improve the nucleic acid sample volume by 20.62μL compared with the constant time dispersion method.Compared with the traditional Sigmoid function model to realize the S-curve acceleration and deceleration control algorithm,the equal frequency discrete method can improve the positioning accuracy of motion and make the extraction process smoother,and effectively improve the sample volume under the oscillating motion of nucleic acid extraction.

关 键 词：步进电机 核酸提取 加减速控制 SIGMOID函数 定位精度 

分 类 号：TP273[自动化与计算机技术—检测技术与自动化装置] TP212[自动化与计算机技术—控制科学与工程] TB9[一般工业技术—计量学]