非小细胞肺癌患者瘤组织LncRNA LINC00460、miR-98-5p的水平及临床意义  

Expression and clinical significance of LncRNA LINC00460 and miR-98-5p in tumor tissues of patients with non-small cell lung cancer

作　　者：王敏[1] 苗玲 赵之寒 WANG Min;MIAO Lin;ZHAO Zhi-han(Department of Chest Surgery,Handan Central Hospital,Handan 056000,Hebei,China)

机构地区：[1]邯郸市中心医院胸外科,河北邯郸056000

出　　处：《广东医学》2023年第11期1368-1373,共6页Guangdong Medical Journal

基　　金：河北省医学科学研究课题(20200194)。

摘　　要：目的 探讨非小细胞肺癌(NSCLC)患者瘤组织长链非编码RNA(lncRNA)LINC00460、microRNA-98-5p(miR-98-5p)表达及临床意义。方法 选取2017年1月至2018年12月57例NSCLC患者病例资料进行回顾性研究。术中留取癌组织和癌旁组织(距肿瘤边缘2 cm),比较癌组织及癌旁组织miR-98-5p、LncRNA LINC00460表达,分析癌组织miR-98-5p、LncRNA LINC00460表达与病理特征的相关性,比较不同预后患者miR-98-5p、LncRNA LINC00460表达,logistic回归分析癌组织miR-98-5p、LncRNA LINC00460表达与预后的关系,并比较癌组织miR-98-5p、LncRNA LINC00460表达不同患者总生存率,列线图分析miR-98-5p、LncRNA LINC00460对患者预后评估价值。结果 癌组织miR-98-5p表达低于癌旁组织,LncRNA LINC00460表达高于癌旁组织(P<0.05)。NSCLC患者癌组织miR-98-5p、LncRNA LINC00460表达与临床分期、分化程度、淋巴结转移有关(P<0.05)。死亡者癌组织miR-98-5p表达较存活者低,LncRNA LINC00460表达较存活者高(P<0.05)。Logistic回归分析显示,癌组织LncRNA LINC00460高表达、miR-98-5p低表达是NSCLC患者预后死亡的独立危险因素(P<0.05)。癌组织LncRNA LINC00460高表达者总生存率低于低表达者,miR-98-5p高表达者总生存率高于低表达者(P<0.05)。构建miR-98-5p、LncRNA LINC00460评估患者预后的列线图预测模型与实际病死风险吻合度较高(χ^(2)=6.902,P=0.547)。结论 NSCLC患者癌组织LncRNA LINC00460异常高表达,miR-98-5p异常低表达,且异常表达程度与患者预后关系密切,可为临床评估预后提供参考。Objective To investigate the expression and clinical significance of long non-coding RNA (lncRNA) LINC00460 and microRNA-98-5p (miR-98-5p) in tumor tissues of patients with non-small cell lung cancer (NSCLC). Methods A retrospective study was conducted using clinical data from 57 NSCLC patients between January 2017 and December 2018. Tumor tissue and adjacent non-tumor tissue (2 cm away from the tumor edge) were collected during surgery. The expression levels of miR-98-5p and LncRNA LINC00460 in tumor and adjacent non-tumor tissues were compared. The correlation between the expression of miR-98-5p, LncRNA LINC00460, and pathological features in tumor tissues was analyzed. The expression of miR-98-5p and LncRNA LINC00460 in tumor tissues was compared between patients with different prognoses. Logistic regression analysis was performed to assess the relationship between the expression of miR-98-5p, LncRNA LINC00460 in tumor tissues, and patient prognosis. The overall survival rates of patients with different expression levels of miR-98-5p and LncRNA LINC00460 in tumor tissues were compared. A line chart analysis was conducted to evaluate the prognostic value of miR-98-5p and LncRNA LINC00460 in patients. Results The expression of miR-98-5p in tumor tissues was lower than that in adjacent non-tumor tissues, while the expression of LncRNA LINC00460 in tumor tissues was higher than that in adjacent non-tumor tissues (P<0.05). The expression of miR-98-5p and LncRNA LINC00460 in tumor tissues of NSCLC patients was associated with clinical stage, differentiation degree, and lymph node metastasis (P<0.05). Deceased patients had lower miR-98-5p expression and higher LncRNA LINC00460 expression in tumor tissues compared to survivors (P<0.05). Logistic regression analysis showed that high expression of LncRNA LINC00460 and low expression of miR-98-5p in tumor tissues were independent risk factors for poor prognosis in NSCLC patients (P<0.05). Patients with high LncRNA LINC00460 expression in tumor tissues had lower overall s

关 键 词：非小细胞肺癌 长链非编码RNA microRNA-98-5p 病理特征 预后 

分 类 号：R734.2[医药卫生—肿瘤] R73[医药卫生—临床医学]