槐耳通过PI3K/AKT信号通路诱导结直肠癌细胞凋亡  

Huaier Inducing Apoptosis Through PI3K/AKT Signaling Pathway in Colorectal Cancer

作　　者：温璐雯 冯琦 霍明一 柳新 程玉[1] 郑纪宁[1] WEN Lu-wen;FENG Qi;HUO Ming-yi;LIU Xin;CHENG Yu;ZHENG Ji-ning(Pathology Department of Chengde Medical University,Chengde,Hebei,067000,China;Basic Medical Institute of Chengde Medical University;Oncology department of Chengde Medical University Affiliated Hospital)

机构地区：[1]承德医学院病理教研室,河北承德067000 [2]承德医学院基础医学研究所 [3]承德医学院附属医院

出　　处：《承德医学院学报》2023年第6期451-455,共5页Journal of Chengde Medical University

基　　金：河北省教育厅科学基金项目培育基金(ZD2020137);河北省高校重点学科建设项目[冀教高(2013)4号]。

摘　　要：目的探讨槐耳能否影响结直肠癌增殖和凋亡及其机制。方法通过细胞增殖实验检测槐耳对结直肠癌HCT8和HT29细胞增殖的影响,流式细胞术检测槐耳对细胞凋亡的影响,RT-qPCR检测槐耳对P85αmRNA表达水平影响,Western blot检测槐耳对PI3K/AKT通路蛋白P85α、p-AKT和AKT,凋亡蛋白BAX、BCL-2、Cleaved caspase-3和Cleaved caspase-9以及增殖蛋白CDK4和Cyclin D1表达量的影响。结果细胞增殖实验结果显示,槐耳能降低HCT8和HT29细胞的存活率,且存活率随浓度增加而递减。不同浓度槐耳作用下HCT8和HT29细胞凋亡率显著升高,即槐耳能促进HCT8和HT29的凋亡,且凋亡率随浓度增加而递增。不同浓度槐耳作用下,HCT8和HT29细胞中P85αmRNA表达水平显著下调,即槐耳能抑制P85α基因表达水平,且其表达水平随浓度增加而降低。Western blot表明,槐耳可显著降低P85α、p-AKT/AKT、BCL-2、CDK4、Cyclin D1的表达量,提高BAX、Cleaved caspase-3、Cleaved caspase-9的表达量(P均<0.05)。结论槐耳能降低结直肠癌HCT8和HT29细胞存活力和促进细胞凋亡,其机制可能与PI3K/AKT信号通路有关。Objective To investigate whether huaier can induce apoptosis of colorectal cancer(CRC)cells and its possible mechanism.Methods CCK8 assays detected the viability of HCT8 and HT29 cells with different concentration of huaier.Flow cytometry detected the apoptosis ofHCT8 and HT29 cells,and the gene expression lever of P85αmRNA was detected by RT-qPCR assays.Western blot assays detected the expression lever of pathway protein P85a,p-AKT and AKT,apoptosis protein BAX,BCL-2,Cleaved caspase-3 and Cleaved caspase-9,and proliferating protein CDK4 and CyclinD1 on HCT8 and HT29 cells with different concentration of huaier.Results Huaier could reduce the viability ofHCT8 and HT29 cells,and the viability were decreasing with the increasing concentration.The apoptosis rates of HCT8 and HT29 cells with different concentration of huaier were increased observably.It showed that huaier could increase the apoptosis rates of HCT8 and HT29 cells,and the apoptosis rates were increasing with the increasing concentration.The expression lever ofP85a mRNA in HCT8 and HT29 cells were decreased observably.It showed that huaier could decrease the expression lever of P85αmRNA,and the expression lever of P85a mRNA were decreasing with the increasing concentration.Westem blot assays showed that huaier can inhibit the expression lever of P85α,p-AKT/AKT,inhibit the expression of BCL-2,CDK4 and CyclinD1,and promote the expression lever of BAX,Cleaved caspase-3 and Cleaved caspase-9.Conclusion Huaier can inhibiting the proliferation and inducing the apoptosisof HCT8 and HT29 cells,and its mechanism may be related with PI3K/AKT signaling pathway.

关 键 词：槐耳 结直肠癌 凋亡 PI3K/AKT信号通路 

分 类 号：R735.3[医药卫生—肿瘤]