染料木黄酮抑制前列腺癌进展和转移的机制研究  

Study on the mechanism of genistein inhibiting the progression and metastasis of prostate cancer

作　　者：刘文瞻[1] 蔡启亮[2] 吴宝军 杨思维 姚智力 侯泽楷 孙彬栩[4] LIU Wenzhan;CAI Qiliang;WU Baojun;YANG Siwei;YAO Zhili;HOU Zekai;SUN Binxu(Department I of Urology,Handan First People's Hospital,Handan 056000,China;Department of Urology,the Second Hospital of Tianjin Medical University;Department of Urology,Jinnan Hospital of Tianjin;Department of Oncology,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine)

机构地区：[1]邯郸市第一医院泌尿一科,056000 [2]天津医科大学第二医院泌尿外科 [3]天津市津南医院泌尿外科 [4]天津中医药大学附属第一医院肿瘤科

出　　处：《天津医药》2023年第12期1288-1292,共5页Tianjin Medical Journal

基　　金：天津市自然科学基金资助项目(21JCYBJC01470);天津市卫生健康委科技人才培育项目(KJ20137);天津市津南区科技计划项目(20161523)。

摘　　要：目的探讨染料木黄酮对前列腺癌细胞增殖、迁移和侵袭能力的影响及其分子机制。方法将前列腺癌细胞株LNCaP和CWR22RV1细胞分为对照组(常规培养)和实验组(50μmol/L染料木黄酮处理)。采用噻唑蓝(MTT)法分析染料木黄酮对前列腺癌细胞增殖能力的影响,通过细胞划痕实验和Transwell实验分析染料木黄酮对前列腺癌细胞迁移及侵袭能力的影响。Western blot检测上皮间质转化(EMT)中间质标志物E-钙黏蛋白(ECadherin)、N-钙黏蛋白(N-Cadherin)、波形蛋白(Vimentin)以及肿瘤干细胞标志物CD44和Oct4的蛋白水平。结果MTT实验结果表明,染料木黄酮具有抑制前列腺癌细胞增殖的作用。实验组LNCaP和CWR22RV1细胞划痕闭合率较对照组下调,穿过Transwell膜的细胞数量减少(P<0.05)。Western blot实验证实,染料木黄酮能够下调前列腺癌细胞中间质的标志物N-Cadherin、Vimentin和肿瘤干细胞的标志物CD44与Oct4蛋白表达,上调上皮细胞标志物ECadherin表达(P<0.01)。结论染料木黄酮通过抑制EMT过程并降低前列腺癌细胞的干性,从而降低前列腺癌细胞的增殖、迁移和侵袭能力。Objective To investigate the effect of genistein on the proliferation,migration and invasion of prostate cancer cells and its molecular mechanism.Methods Prostate cancer LNCaP and CWR22RV1 cells were divided into the control group(conventional culture)and the experimental group(50μmol/L genistein treatment).The effect of genistein on the proliferation of prostate cancer cells were analyzed by MTT assay.The effect of genistein on the migration and invasion of prostate cancer cells were analyzed by cell scratch assay and Transwell assay.The protein levels of epithelial interstital transformation(EMT)intermediate markers E-Cadherin,N-Cadherin,Vimentin,and tumor stem cell markers CD44 and Oct-4 were detected by Western blot assay.Results MTT assay showed that genistein could inhibit the proliferation of prostate cancer cells.The scratch closure rates of LNCaP and CWR22RV1 cells were significantly reduced in the experimental group compared with those in the control group,and the number of cells passing through the Transwell membrane was significantly reduced(P<0.05).Western blot assay showed that genistein could down-regulate the expression levels of N-Cadherin,Vimentin,CD44 and Oct4 in prostate cancer cells,and up-regulate the expression of ECadherin in epithelial cells(P<0.01).Conclusion Genistein reduces the dryness of prostate cancer cells by inhibiting the EMT process,thus reducing the proliferation,migration and invasion of prostate cancer cells.

关 键 词：前列腺肿瘤 染料木黄酮 上皮-间质转化 细胞运动 

分 类 号：R737.25[医药卫生—肿瘤]