Wide grain 4,encoding an alpha-tubulin,regulates grain size by affecting cell expansion in rice  

作　　者：Yi Liu Lianan Guo Guoli Qu Yang Xiang Xu Zhao Hua Yuan Ting Li Liangzhu Kang Shiwen Tang Bin Tu Bingtian Ma Yuping Wang Shigui Li Weilan Chen Peng Qin 

机构地区：[1]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Rice Research Institute,Sichuan Agricultural University,Chengdu 611130,Sichuan,China

出　　处：《The Crop Journal》2023年第6期1931-1936,共6页作物学报（英文版）

基　　金：the National Natural Science Foundation of China(32121003),the Sichuan Science and Technology Program(2022ZDZX0012,2022YFQ0026);the Natural Science Foundation of Sichuan province(2022NSFSC1667).

摘　　要：Rice is one of the three most important food crops in the world.Increasing rice yield is an effective way to ensure food security.Grain size is a key factor affecting rice yield;however,the genetic and molecular mechanisms regulating grain size have not been fully investigated.In this study,we identified a rice mutant,wide grain 4-D(wg4-D),that exhibited a significant increase in grain width and a decrease in grain length.Histological analysis demonstrated that WG4 affects cell expansion thereby regulating grain size.MutMap-based gene mapping and complementary transgenic experiments revealed that WG4 encodes an alpha-tubulin,OsTubA1.A SNP mutation in WG4 affected the arrangement of cortical microtubules and caused a wide-grain phenotype.WG4 is located in nuclei and cytoplasm and expressed in various tissues.Our results provide insights into the function of tubulin in rice and identifies novel targets the regulation of grain size in crop breeding.

关 键 词：Cell size MICROTUBULES Oryza sativa WG4 

分 类 号：S511[农业科学—作物学]