免疫亲和柱净化柱后光化学衍生高效液相色谱-荧光检测法同时测定制马肾中4种黄曲霉毒素  

Simultaneous Determination of Content of Four Aflatoxins in EQUI PENIS ET TESTIVULUS by Mmunoaffinity Column Clean-up and HPLC-FLD with Post-column Photochemical Derivatization

作　　者：孙艳杰[1] 赵磊[1] 李正刚 王路宏[1] SUN Yanjie;ZHAO Lei;LI Zhenggang;WANG Luhong(Siping Institute for Food and Drug Control,Siping 13600,China)

机构地区：[1]吉林省四平市食品药品检验所,吉林四平136000

出　　处：《中国民族民间医药》2023年第23期19-22,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：吉林省地方中药炮制规范项目(JLPZGF-2020-066)。

摘　　要：目的:建立免疫亲和柱净化柱后光化学衍生高效液相色谱-荧光检测法同时测定制马肾中黄曲霉毒素B_(1)、B_(2)、G_(1)、G_(2)的含量。方法:样品采用70%甲醇作为提取溶剂,经免疫亲和柱净化、高效液相色谱分离、光化学柱后衍生后,通过荧光检测器测定其中黄曲霉毒素的含量。结果:黄曲霉毒素B_(1)的线性范围为0.0104~0.0520 ng(r=0.9999)、黄曲霉毒素B_(2)的线性范围为0.0038~0.0190 ng(r=0.9998)、黄曲霉毒素G_(1)的线性范围为0.0108~0.0540 ng(r=0.9998)、黄曲霉毒素G_(2)的线性范围为0.0038~0.0190 ng(r=0.9998),线性关系良好,回收率在89.68%~101.44%之间,RSD≤3.5%。结论:该方法操作简便,灵敏度高、重复性好、结果准确,可用于制马肾中黄曲霉毒素的测定。Objective To establish the contamination method of aflatoxin B_(1),B_(2),G_(1)and G_(2)in EQUI PENIS ET TESTIVULUS by immunoaffinity column clean-up and HPLC-FLD with post-column photochemical derivatization.Methods After extraction with 70%Methanol and purification by immunoaffinity columns,aflatoxin B_(1),B_(2),G_(1)and G_(2)in samples were anlyzed by HPLC-FLD with post-column photochemical derivatization.Results The linearity of Aflatoxin B_(1)was at 0.0104-0.0520 ng(r=0.9999),Aflatoxin B_(2)was at 0.0038-0.0190 ng(r=0.9998),Aflatoxin G_(1)was at 0.0108-0.0540 ng(r=0.9998),Aflatoxin G_(2)was at 0.0038~0.0190 ng(r=0.9998),The average recoveries were within 89.68%-101.44%with RSD≤3.5%.Conclusion The above method has demonstrated convenient operation,good repeatability,highsensitity and accuracy.This method is suitable for the determination of AF in Renshenguipi pills.

关 键 词：制马肾 免疫亲和柱 光化学衍生 高效液相色谱-荧光检测器 黄曲霉毒素 

分 类 号：R284.1[医药卫生—中药学]