重症龋患儿唾液TRPA1、NLRP3的表达水平及临床意义  

Expression levels of TRPA1 and NLRP3 in the saliva of children with severe caries and its clinical significance

作　　者：李姣 郭战坤[1] 魏鹏 李雪莉[1] 李燕 LI Jiao;GUO Zhankun;WEI Peng;LI Xuei;LI Yan(Department of Stomatology,Baoding Maternal and Child Health Hospital,Baoding,Hebei,China,071000;Department of Internal Medicine,Jingxiu District Hospital,Baoding City,Baoding,Hebei,China,071000)

机构地区：[1]保定市妇幼保健院口腔科,河北保定071000 [2]保定市竞秀区医院内科,河北保定071000

出　　处：《分子诊断与治疗杂志》2023年第10期1833-1836,1850,共5页Journal of Molecular Diagnostics and Therapy

基　　金：保定市科技计划项目(2041ZF139)。

摘　　要：目的 研究重症龋患儿唾液瞬时电位受体离子通道A1(TRPA1)、Nod样受体蛋白3(NLRP3)的表达水平及临床意义。方法 选择2021年1月至2022年6月期间在保定市妇幼保健院接受治疗的142例龋齿患儿,根据龋失补指数(DMFT)分为重症组(n=46)和非重症组(n=96);另取同期体检的70名无龋齿健康儿童作为对照组(n=70)。检测并比较三组受试儿童唾液TRPA1、NLRP3表达水平的差异,采用Pearson检验分析TRPA1、NLRP3表达水平与DMFT的相关性。采用受试者工作特征(ROC)曲线分析唾液TRPA1、NLRP3表达水平对重症龋的诊断价值,采用单因素分析和多因素logistic分析探讨重症龋的影响因素。结果 三组唾液TRPA1、NLRP3表达水平比较为:重症组>非重症组>对照组,差异均有统计学意义(F=75.482,91.391,P<0.05);重症组患儿唾液TRPA1、NLRP3的表达水平与DMFT呈正相关(r=0.351、0.384,P<0.05);唾液TRPA1、NLRP3的表达水平对重症龋具有诊断价值,截断值分别为1.41 pg/mL、2.54 pg/mL,灵敏度分别为76.09%、89.13%,特异度分别为83.33%、82.29%;吃甜食频率≥2次/d、唾液TRPA1表达水平≥1.41 pg/mL、唾液NLRP3表达水平≥2.54 pg/mL是发生重症龋的危险因素,刷牙频率≥2次/天、家长监督刷牙是发生重症龋的保护因素(P<0.05)。结论 重症龋发病可能与TRPA1、NLRP3表达增加介导的生物学效应相关。Objective To investigate the expression levels of transient receptor potential A1(TRPA1)and Nod-like receptor 3(NLRP3)in the saliva of children with severe caries and its clinical significance.Methods A total of 142 children with dental caries who were treated in Baoding Maternal and Child Health Hospital from January 2021 to June 2022 were selected and divided into two groups based on the decayed-mising-filled teeth(DMFT)index:a severe group(n=46)and a non-severe group(n=96).Additionally,another 70 healthy children without dental caries were cchosen as the control group(n=70).The expression levels of TRPA1 and NLRP3 in the saliva were detected and compared among the three groups,and the correlation between TRPA1 and NLRP3 expression levels and DMFT was analyzed using the Pearson test.The expression levels of TRPA1 and NLRP3 in saliva were analyzed using the receiver operating characteristic(ROC)curves to determine their value for severe caries.The influence factors of severe caries were analyzed by univariate analysis and multiple logistic analysis.Results The comparison of the expression levels of TRPA1 and NLRP3 in saliva among three groups were the severe group having>the non-severe group>the control group,with statistically significant(F=75.482,91.391,P<0.05).The expression levels of TRPA1 and NLRP3 in the saliva of severe children were positively correlated with DMFT(r=0.351,0.384,P<0.05).The expression levels of TRPA1 and NLRP3 in saliva had diagnostic value for severe caries,with cut-off values of 1.41 pg/mL and 2.54 pg/mL,respectively;the sensitivity was 76.09%for TRPA1 and 89.13%for NLRP3 while the specificity was 83.33%for TRPA1 and 82.29%for NLRP3.The risk factors associated with the development of severe caries included a frequency of eating sweets≥2 times/day,an expression level of TRPA1 in saliva≥1.41 pg/mL,and an expression level of NLRP3 in saliva≥2.54 pg/mL(P<0.05).On the other hand,brushing teeth≥2 times/day and parental supervision were identified as protective factors against the deve

关 键 词：重症龋 瞬时电位受体离子通道A1 Nod样受体蛋白3 龋失补指数 

分 类 号：R788.1[医药卫生—口腔医学]