SFRP1对头颈鳞状细胞癌增殖和侵袭的影响及其机制研究  

Effect of SFRP1 on the proliferation and invasion of head and neck squamous cell carcinoma and its mechanism

作　　者：杨中玉 李薇[1] 朱光正 赵文懿 徐静[1] YANG Zhongyu;LI Wei;ZHU Guangzheng;ZHAO Wenyi;XU Jing(Department of Plastic Surgery,the First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui 233004,China;不详)

机构地区：[1]蚌埠医学院第一附属医院整形外科,安徽蚌埠233004 [2]蚌埠医学院组织移植实验室,安徽蚌埠233000

出　　处：《中华全科医学》2023年第12期2040-2044,共5页Chinese Journal of General Practice

基　　金：安徽高校自然科学研究项目(KJ2020A0576);蚌埠医学院科技项目(2020byzd137);蚌埠医学院研究生科研创新计划项目(Byycxz21086)。

摘　　要：目的通过研究分泌型卷曲相关蛋白1(SFRP1)对头颈鳞状细胞癌(HNSC)增殖、迁移的影响及机制,观测头颈鳞状细胞癌在体外的生物学活动,对治疗头颈鳞状细胞癌提出新的治疗方案。方法使用生物信息学分析SFRP1在肿瘤中的表达水平及与肿瘤侵袭和增殖的相关性,研究头颈鳞状细胞癌与SFRP1的关系。将头颈鳞状细胞癌细胞SCL-1利用转染敲低SFRP1的表达量,使用干扰SFRP1组(small interfering RNA)和对照组(si-NC)进行CCK-8实验分析、划痕实验分析和Transwell实验分析,分别检测细胞增殖、迁移情况。通过Western blotting实验检测SCL-1细胞中Wnt/β-catenin信号转导通路及C-myc、cyclinD1的表达水平。结果与对照组头颈鳞状癌细胞相比,皮肤癌细胞SFRP1蛋白表达量降低(P<0.01);对照组头颈鳞状细胞癌组在24 h迁移面积比为0.602±0.019,而空白组为0.419±0.053、对照组为0.435±0.009,si-SFRP1组迁移能力明显高于对照组和空白组(P<0.01);在Transwell实验中,24 h后si-SFRP1组侵袭的细胞数为(723.333±2.048)个,而空白组为(332.002±9.930)个、对照组为(343.332±32.504)个,si-SFRP1组侵袭能力明显高于对照组和空白组(P<0.01);SCL-1中敲低SFRP1通过Wnt/β-catenin及下游C-myc、cyclinD1表达水平升高(P<0.01)。通过生物信息学数据统计分析,发现敲低SFRP1蛋白,可促进头颈鳞状细胞癌的增殖,对临床分析有一定的指导作用。结论SFRP1在头颈鳞状细胞癌细胞内低表达,同时通过Wnt/β-catenin信号转导通路促进细胞的增殖和迁移能力。Objective To observe the biological activities of head and neck squamous cell carcinoma(HNSC)in vitro by studying the proliferation,migration and mechanism of secreted frizzled related protein 1(SFRP1),and to propose new therapeutic options for the treatment of HNSC.Methods The association of SFRP1 with head and neck squamous cell carcinoma was investigated using bioinformatics analysis of the correlation between SFRP1 expression levels in tumors and tumor invasion and proliferation.Skin cancer cells SCL-1 were knocked down the expression of SFRP1 using transfection,and the effect of cell proliferation,migration was examined using interference with SFRP1 group(si-SFRP1)and control group(si-NC)for CCK-8 assay analysis,scratch assay analysis and Transwell assay analysis,respectively.The Wnt/β-catenin signaling pathway and the expression levels of C-myc and cyclinD1 in SCL-1 cells were detected by Western blotting assay.Results Compared with the control head and neck squamous carcinoma cells,skin cancer cells were found to be low expressing SFRP1 protein(P<0.010);the migration area ratio of the low expressing SFRP1 protein neck squamous cell carcinoma group was 0.602±0.019 at 24 hours,compared with 0.419±0.053 in the blank group and 0.435±0.009 in the control group,and the migration ability of the si-SFRP1 group was significantly higher than that of the control and blank groups(P<0.01);in the Transwell experiment,the number of cells invaded by the si-SFRP1 group after 24 hours was 723.333±2.048,compared with 332.002±9.930 in the blank group and 343.332±32.504 in the control group,and the invasion ability of the si-SFRP1 group was significantly higher than that of the control and blank group(P<0.01);knockdown of SFRP1 in SCL-1 was elevated through Wnt/β-catenin and downstream C-myc and cyclinD1 expression levels(P<0.01).Statistical analysis of bioinformatics data revealed that knockdown of SFRP1 protein had promoted the proliferation of head and neck squamous cell carcinoma,which is a guide for clinical analy

关 键 词：分泌型卷曲相关蛋白1 头颈鳞状细胞癌 WNT/Β-CATENIN信号转导通路 肿瘤转移 

分 类 号：R739.91[医药卫生—肿瘤] R730.43[医药卫生—临床医学]