P16/Ki67免疫化学染色在宫颈癌高危人群细胞学与组织学诊断中的应用价值  

Application value of P16/Ki67 immunochemical staining in cytological and histological diagnosis of cervical cancer in high risk population

作　　者：孙文燕 赖红梅 肖名珍 邵云蕾 赖超 李丽 邱晶 SUNWenyan;LAI Hongmei;XIAO Mingzhen;SHAO Yunlei;LAI Chao;LI Li;QIU Jing(Department of Pathology,Nankang District First People's Hospital of Ganzhou City,Ganzhou,Jiangxi,341400,China;Department of Pathology,Ganxian District People's Hospital of Ganzhou City,Ganzhou,Jiangxi,341100,China)

机构地区：[1]赣州市南康区第一人民医院病理科,江西赣州341400 [2]赣州市赣县区人民医院病理科,江西赣州341100

出　　处：《当代医学》2023年第15期125-128,共4页Contemporary Medicine

基　　金：赣州市指导性科技计划项目(GZ2021ZSF454)。

摘　　要：目的探讨P16/Ki67免疫化学染色在宫颈癌高危人群细胞学与组织学诊断中的应用价值。方法选取2020年1月至2021年10月本院收治的80例宫颈癌高危人群患者的宫颈脱落细胞及宫颈活体组织作为研究对象。分别进行薄式液基细胞学制片巴氏染色、组织石蜡切片HE染色,两种标本均于显微镜下进行形态观察,两种标本再进行P16/Ki67免疫化学染色。综合细胞形态学、组织形态学及P16/Ki67免疫化学染色检测结果为病理结果,以病理结果为金标准。分析80例宫颈癌高危人群患者的病理检查结果;统计细胞学形态学和组织形态学的阳性检出率及诊断符合率;细胞形态学、组织形态学分别联合P16/Ki67免疫化学染色结果;比较细胞形态学与细胞形态学联合P16/Ki67免疫化学染色结果、组织形态学与组织形态学联合P16/Ki67免疫化学染色结果。结果病理结果显示,宫颈慢性炎7例、宫颈上皮内瘤变(CIN)130例、CIN223例、CIN312例、宫颈癌8例。细胞学形态学结果显示,阳性45例、阴性35例,阳性检出率为56.25%,诊断符合率为87.50%;组织形态学初诊结果显示,阳性42例、阴性38例,阳性检出率为52.50%,诊断符合率为93.75%。细胞形态学联合P16/Ki67免疫化学染色结果显示,阳性40例、阴性40例,阳性率为50.00%,诊断符合率为93.75%;组织形态学联合P16/Ki67免疫化学染色结果显示,阳性41例、阴性39例,阳性检出率为51.25%,诊断符合率为97.50%。细胞形态学阳性检出率高于细胞形态学联合P16/Ki67免疫化学染色,诊断符合率低于细胞形态学联合P16/Ki67免疫化学染色,但差异无统计学意义;组织形态学阳性检出率高于组织形态学联合P16/Ki67免疫化学染色,诊断符合率低于组织形态学联合P16/Ki67免疫化学染色,但差异无统计学意义。结论P16和Ki67免疫化学染色应用于宫颈癌高危人群中可提高宫颈高级别上皮内病变诊断准确性,减少误诊和漏Objective To investigate the application value of P16/Ki67 immunochemical staining in cytological and histological diagnosis of cervical cancer in high risk population.Methods Cervical exfoliated cells and cervical living tissues of 80 patients with high risk of cervical cancer admitted to our hospital from January 2020 to October 2021 were selected as the study subjects.Thin liquid-based cytology was performed with Pap staining and paraffin HE staining.Morphology of both specimens was observed under microscope,and P16/Ki67 immunochemical staining was performed on both specimens.The results of cell morphology,histomorphology and P16/Ki67 immunohistochemical staining were pathological results,with pathological results as the gold standard.Pathological examination results of 80 patients with high risk of cervical cancer were analyzed;the positive detection rate and diagnostic coincidence rate of cytology morphology and histomorphology were counted;the results of cell morphology and histomorphology combined with P16/Ki67 immunohistochemical staining were obtained;the results of cell morphology and cell morphology combined with P16/Ki67 immunohistochemical staining and the results of histomorphology and histomorphology combined with P16/Ki67 immunohistochemical staining were compared.Results The pathological results showed that there were 7 cases of chronic cervical inflammation,30 cases of cervical intraepithelial neoplasia(CIN)1,23 cases of CIN2,12 cases of CIN3 and 8 cases of cervical cancer.Cytological morphology showed that 45 cases were positive and 35 were negative,the positive detection rate was 56.25%,and the diagnostic coincidence rate was 87.50%;the initial histological results showed that 42 cases were positive,38 were negative,the positive detection rate was 52.50%,and the diagnostic coincidence rate was 93.75%.The results of cell morphology combined with P16/Ki67 immunohistochemical staining showed that 40 cases were positive,40 cases were negative,the positive rate was 50.00%,and the diagnostic coincid

关 键 词：宫颈癌 高危人群 P16/Ki67免疫化学染色 高级别鳞状上皮内病变 

分 类 号：R737.33[医药卫生—肿瘤]