脂滴包被蛋白在非酒精性脂肪性肝病小鼠肝组织中的表达谱及瘦素基因敲除对其的影响  

Expression profile of Perilipins in liver tissue of mice with non-alcoholic fatty liver disease and the effect of leptin gene knockout on it

作　　者：于庆红 康碧莲 孙祥云 齐一菲 刘琳[1] 丛敏[1] 王萍[1] 刘天会[1] YU Qing-hong;KANG Bi-lian;SUN Xiang-yun(Liver Research Center,Beijing Friendship Hospital,Capital Medical University,Beijing Key Laboratory of Translational Medicine in Liver Cirrhosis,National Clinical Research Center of Digestive Diseases,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院肝病中心肝硬化转化医学北京市重点实验室暨国家消化系统疾病临床医学研究中心,北京100050

出　　处：《临床和实验医学杂志》2023年第21期2241-2244,共4页Journal of Clinical and Experimental Medicine

基　　金：国家自然科学基金(编号:82070618,81770596)。

摘　　要：目的探讨不同饮食诱导的非酒精性脂肪肝病(NAFLD)小鼠肝组织中脂滴包被蛋白(Plin)家族的表达谱及瘦素基因敲除对其的影响。方法实验选用8周龄雄性C57BL/6小鼠53只,按照随机数字表法分组:CDAA对照组和模型组(9周组和15周组)、WD对照组和模型组(2周组、6周组和30周组),除CDAA9周对照组为5只,其余每组6只。选用4周龄雄性ob/ob小鼠12只,按照随机数字表法分组:对照组和模型组,每组6只。对照组给予正常饮食喂养;分别采用胆碱缺乏、L-氨基酸替代蛋白饲料(CDAA)和西方饮食(WD)诱导建立野生型C57BL/6小鼠NAFLD模型;采用WD饮食诱导建立瘦素基因敲除小鼠(ob/ob)NAFLD模型。实时荧光定量PCR法检测肝组织中Plin 2,Plin 3,Plin 4,Plin 5 mRNA的表达,比较不同组之间的差异及瘦素敲除对其的影响。结果正常野生型C57BL/6小鼠的肝组织中可检测出Plin 2,Plin 3,Plin 4,Plin 5四种亚型mRNA的表达;其中Plin 2是主要的亚型,其次为Plin 3、Plin 5,Plin 4的表达量最低。在CDAA饮食诱导的NAFLD模型中,Plin 2表达无显著变化;Plin 3表达在造模15周降低(P<0.05);Plin 4表达在造模9周和15周均升高(P<0.05);Plin 5表达在造模9周和15周均降低(P<0.05)。在WD饮食诱导的NAFLD模型中,野生型小鼠肝组织中Plin 2表达在造模30周增加(P<0.05);Plin 3、5表达无显著变化;Plin 4表达在造模2周、6周和30周均升高(P<0.05)。与野生型小鼠不同,在WD饮食诱导的(ob/ob)小鼠NAFLD模型中,肝脏中Plin 2、Plin 3、Plin 5表达降低(P<0.05);而Plin 4表达无显著变化。结论Plins基因表达谱在不同饮食诱导的NAFLD小鼠肝组织中存在显著差异;瘦素基因敲除抑制NAFLD小鼠肝组织中Plins基因的表达。Objective To investigate the expression profile of the Perilipin(Plin)family and the effect of leptin gene knockout on Plins in the liver tissues of non-alcoholic fatty liver disease(NAFLD)models induced by different diets.Methods A total of 538-week-old male C57BL/6 mice were selected and divided into CDAA control group and model group(9-week group and 15-week group),WD control group and model group(2-week group,6-week group and 30-week group).There were 6 mice in each group except the CDAA 9-week control group(n=5).Twelve 4-week-old male ob/ob mice were divided into control group and model group according to the random number table method,with 6 mice in each group.The control group was fed normal diet,NAFLD models in wild-type mice were induced by choline-deficient,L-amino acid-defined(CDAA)diet and western diet(WD),respectively.NAFLD model in leptin gene knockout mice(ob/ob)was induced by WD.Real-time PCR was used to detect the expression of Plin 1-5 in liver tissues.The differences of Plin 2,Plin 3,Plin 4,Plin 5 expression in different groups and the effects of leptin knockout on Plin 2,Plin 3,Plin 4,Plin 5 were analyzed.Results Plin 2,Plin 3,Plin 4,Plin 5 mRNA were detected in normal liver tissue of wild type mice.Plin 2 was the most dominant subtype,followed by Plin 3 and Plin 5,and the expression of Plin 4 was the lowest.In the CDAA-induced NAFLD model,there was no significant change in Plin 2.The expression of Plin 3 decreased at 15 weeks after modeling(P<0.05).The expression of Plin 4 increased at 9 weeks and 15weeks(P<0.05).The expression of Plin 5 decreased at 9 weeks and 15 weeks(P<0.05).In the WD diet-induced NAFLD model,the expression of Plin 2 in liver tissue of wild-type mice increased at 30 weeks after modeling(P<0.05).There were no significant changes in Plin 3 and Plin 5.The expression of Plin 4 increased at 2 weeks,6weeks and 30weeks(P<0.05).Different from wild-type mice,the expressions of Plin 2,Plin 3 and Plin 5 in liver of WD diet-induced(ob/ob)mice in NAFLD model decreased(P<0.05).The expr

关 键 词：小鼠 脂滴包被蛋白 非酒精性脂肪性肝病 瘦素 

分 类 号：R575.5[医药卫生—消化系统]