晒田环境胁迫对克氏原螯虾卵巢发育相关生化指标和激素的影响  

Effects of sun-field environmental stress on biochemical indexes and hormones related to ovarian development of Procambarus clarkii

作　　者：袁畅 李蔚 黄玉英 何苹萍 郑玉斯 李鑫 卢智发 张圣杰 李文红[2] 彭金霞 王大鹏 YUAN Chang;LI Wei;HUANG Yu-ying;HE Ping-ping;ZHENG Yu-si;LI Xin;LU Zhi-fa;ZHANG Sheng-jie;LI Wen-hong;PENG Jin-xia;WANG Da-peng(Guangxi Academy of Fishery Sciences/Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Breeding,Nanning 530021,China;College of Animal Science and Technology,Guangxi University,Nanning 530004,China)

机构地区：[1]广西水产科学研究院/广西水产遗传育种与健康养殖重点实验室,南宁530021 [2]广西大学动物科学技术学院,南宁530004

出　　处：《西南农业学报》2023年第10期2292-2300,共9页Southwest China Journal of Agricultural Sciences

基　　金：广西创新驱动发展专项(桂科AA20302019-3);广西农业农村厅科技自筹经费项目(Z2022100);国家现代农业产业技术体系广西创新团队建设专项资金(nycytxgxcxtd-20-02-2)。

摘　　要：【目的】探究晒田环境胁迫下克氏原螯虾卵巢发育相关生化指标和激素的变化及对卵巢发育的影响,为晒田环境胁迫促进克氏原螯虾卵巢成熟这一生产操作的改良和标准化提供理论依据,促进克氏原螯虾产业可持续发展。【方法】将2个面积为1300 m2的池塘中投放的克氏原螯虾分别作为晒田组和对照组,以晒田组排干水为试验起点,晒田组排水晒田10 d,对照组正常养殖。分别在晒田环境胁迫0、3、7、10 d采集2组中的雌性克氏原螯虾,每次采样数量为30只,测量、称重后取卵巢、肝胰腺和血清,检测卵巢发育相关的生化指标[碱性磷酸酶(AKP/ALP)、酸性磷酸酶(ACP)、谷胱甘肽过氧化物酶(GSH-Px)、前列腺素F2α(PGF2α)、前列腺素E2(PGE2)]、性类固醇激素[雌酮(Estrone)、17β-雌二醇(17β-E2)、睾酮(T)、孕酮(PROG)]、高血糖家族激素[高血糖激素(CHH)、性腺抑制激素(GIH)、蜕皮抑制激素(MIH)、大颚器抑制激素(MOIH)]和卵巢发育相关激素[蜕皮激素(Ecdysone)、甲基法尼酯(MF)、皮质醇(Cortisol)],并进行对比分析。【结果】随着时间增加,晒田组雌性克氏原螯虾卵巢成熟率高于对照组,卵巢发育分期主要分布在IV期。生化指标试验结果表明,晒田胁迫0 d时,晒田组雌性克氏原螯虾不同组织中AKP/ALP、ACP、GSH-Px、PGF2α和PGE2含量均显著低于对照组(P<0.05,下同)或与对照组雌性克氏原螯虾无显著差异(P>0.05,下同);晒田胁迫3和7 d时,晒田组雌性克氏原螯虾不同组织中生化指标含量均显著高于对照组或与对照组雌性克氏原螯虾无显著差异;晒田胁迫10 d时,晒田组雌性克氏原螯虾不同组织中生化指标含量均显著高于对照组雌性克氏原螯虾。性类固醇激素试验结果表明,晒田胁迫0和3 d时,晒田组雌性克氏原螯虾血清中Estrone、17β-E2、T和PROG含量显著高于对照组或与对照组雌性克氏原螯虾无显著差异;晒田胁迫7和10 d【Objective】The purpose of the study was to investigate the changes of biochemical indexes and hormones related to the ovarian development of Procambarus clarkii under the sun-field environmental stress and the effects on ovarian development,to provide theoretical basis for the improvement and standardization of the production operation of sun-field environmental stress to promote the maturation of the ovary of P.clarkii,and to promote the sustainable development of the P.clarkii industry.【Method】P.clarkii were palced in 2 ponds with an area of 1300 m~2 as the sun-field group and the control group.Using draining in the sun-field group as the starting point of the experiment,the sun-field group was drained for 10 days and the control group was cultured normally.Female P.clarkii in the 2 groups were collected at 0,3,7 and 10 days of sun-field environmental stress,respectively.The number of samples was 30 at each time,and the ovaries,hepatopancreas and serum were measured and weighed for the detection of biochemical indices related to ovarian development[alkaline phosphatase(AKP/ALP),acid phosphatase(ACP),glutathione peroxidase(GSH-Px),prostaglandin F2α(PGF2α),prostaglandin E2(PGE2)],sex steroid hormones[Estrone,17β-estradiol(17β-E2),testosterone(T),progesterone(PROG)],the hyperglycemic family hormones[chromogranulin(CHH),gonadal inhibitory hormone(GIH),molting inhibitory hormone(MIH),mandibular organ inhibitory hormone(MOIH)],and the hormones related to ovarian development[Ecdysone,methylfarnesylate(MF),Cortisol]and were comparatively analyzed.【Result】With the increase of time,the ovarian maturation rate of female P.clarkii in the sun-field group was higher than that of the control group,and the ovarian developmental stage was mainly distributed in stage IV.The results of the biochemical index test showed that at 0 days of sun-field stress,the contents of AKP/ALP,ACP,GSH-Px,PGF2αand PGE2 in different tissues of the sun-field group were significantly lower than those of the control group(P<0.05,the s

关 键 词：克氏原螯虾 晒田环境胁迫 生化指标 激素 卵巢发育 

分 类 号：S966.12[农业科学—水产养殖]