重症坏死性胰腺炎患者血清lncRNA MEG3、miR-195-5p表达与病情严重程度及预后的关系  

Relationship between serum lncRNA MEG3 and miR-195-5p expression and disease severity and prognosis in patients with severe necrotizing pancreatitis

作　　者：刘风浩 周真真 李学峰[1] 张旭东 马忠广 LIU Fenghao;ZHOU Zhenzhen;LI Xuefeng;ZHANG Xudong;MA Zhongguang(Second Department of Hepatobiliary and Pancreatic Surgery,Cangzhou Central Hospital,Cangzhou,Hebei 061000,China;Daytime Operating Room,Cangzhou Central Hospital,Cangzhou,Hebei 061000,China;Department of General Surgery,Yanshan People′s Hospital,Cangzhou,Hebei 061399,China)

机构地区：[1]沧州市中心医院肝胆胰外二科,河北沧州061000 [2]沧州市中心医院日间手术室,河北沧州061000 [3]盐山县人民医院普外科,河北沧州061399

出　　处：《国际检验医学杂志》2023年第24期3021-3026,共6页International Journal of Laboratory Medicine

基　　金：河北省医学科学研究课题(20232112)。

摘　　要：目的探讨血清长链非编码RNA MEG3、微小RNA(miR)-195-5p在重症坏死性胰腺炎患者中的表达及其与病情严重程度及预后的关系。方法选取2020年10月至2023年1月沧州市中心医院收治的122例急性胰腺炎患者为研究对象,根据病情严重程度将其分为重症坏死性胰腺炎患者(重症组,53例)和非重症坏死性胰腺炎(非重症组,69例),另根据预后结局将重症坏死性胰腺炎患者分为预后良好组(38例)和预后不良组(15例)。同时选取同期在该院的体检健康者50例为对照组。比较各组临床资料及血清lncRNA MEG3、miR-195-5p水平;采用Spearman相关分析血清lncRNA MEG3、miR-195-5p水平与急性生理学和慢性健康状况Ⅱ(APACHEⅡ)、Ranson评分的关系;采用多因素Logistic回归分析重症坏死性胰腺炎患者发生预后不良的影响因素;采用受试者工作特征(ROC)曲线分析lncRNA MEG3、miR-195-5p对重症坏死性胰腺炎患者预后的评估价值。结果重症组和非重症组年龄、性别、体重指数、基础疾病及病因比较,差异无统计学意义(P>0.05),与非重症组比较,重症组APACHEⅡ、Ranson评分升高(P<0.05);与对照组比较,非重症组和重症组血清lncRNA MEG3、miR-195-5p水平降低(P<0.05),且重症组血清lncRNA MEG3、miR-195-5p水平低于非重症组(P<0.05);Spearman相关分析结果表明,AP患者血清lncRNA MEG3、miR-195-5p水平与APACHEⅡ、Ranson评分均呈负相关(P<0.05);多因素Logistic回归分析结果显示,APACHEⅡ、Ranson评分及血清lncRNA MEG3、miR-195-5p水平是影响重症坏死性胰腺炎患者预后不良的独立危险因素(P<0.05);ROC曲线结果显示,lncRNA MEG3、miR-195-5p单独评估重症坏死性胰腺炎患者预后不良的曲线下面积(AUC)分别为0.767、0.777,灵敏度分别为86.7%、80.0%,特异度分别为49.9%、45.8%,二者联合评估的AUC为0.982,灵敏度、特异度分别为86.7%、78.8%。结论血清lncRNA MEG3、miR-195-5p水平与重症坏死性胰腺炎病情�Objective To investigate the expression of serum long non-coding RNA MEG3 and microRNA(miR)-195-5p in patients with severe necrotizing pancreatitis and their relationship with the severity and prognosis of severe necrotizing pancreatitis.Methods A total of 122 patients with acute pancreatitis admitted to Cangzhou Central Hospital from October 2020 to January 2023 were selected as the research objects.According to the severity of the disease,the patients were divided into severe necrotizing pancreatitis(severe group,53 cases)and non-severe necrotizing pancreatitis(non-severe group,69 cases).According to the prognosis of alternate ending with severe necrotizing pancreatitis can be divided into good prognosis group(38 cases)and poor prognosis group(15 cases).At the same time,50 healthy people who underwent physical examination in the hospital during the same period were selected as the control group.The clinical data and serum levels of lncRNA MEG3 and miR-195-5p in each group were compared.Spearman correlation analysis was used to analyze the relationship between serum levels of lncRNA MEG3,miR-195-5p and acute physiology and chronic health evaluationⅡ(APACHEⅡ)and Ranson scores.Multivariate Logistic regression was used to analyze the influencing factors of poor prognosis in patients with severe necrotizing pancreatitis.The receiver operating characteristic(ROC)curve was used to analyze the value of lncRNA MEG3 and miR-195-5p in evaluating the prognosis of patients with severe necrotizing pancreatitis.Results There was no significant difference in age,gender,body mass index,underlying disease and etiology between severe group and non-severe group(P>0.05).Compared with the non-severe group,APACHEⅡand Ranson scores were significantly increased in the severe group(P<0.05).Compared with the control group,the serum levels of lncRNA MEG3 and miR-195-5p in the non-severe group and the severe group were decreased(P<0.05),and the serum levels of lncRNA MEG3 and miR-195-5p in the severe group were lower than those in th

关 键 词：重症坏死性胰腺炎 长链非编码RNA MEG3 微小RNA-195-5p 

分 类 号：R576[医药卫生—消化系统]