产脂肪酶菌株的筛选及鉴定  

Screening and Identification of Lipase-producing Strains

作　　者：陈美莲 白睿璇 林琳[1,2] CHEN Mei-lian;BAI Rui-xuan;LIN Lin(College of Materials and Chemical Engineering,Minjiang University,Fuzhou,Fujian 350108,China;Institute of Oceanography,Minjiang University,Fuzhou,Fujian 350108,China)

机构地区：[1]闽江学院材料与化学工程学院,福建福州350108 [2]闽江学院海洋研究院,福建福州350108

出　　处：《福建农业科技》2023年第9期63-68,共6页Fujian Agricultural Science and Technology

基　　金：福建省教育厅中青年教师教育科研项目(JAT190627);福建省自然科学基金项目(2020J05177)。

摘　　要：为了挖掘更多的产脂肪酶菌种资源,通过橄榄油平板检测(透明圈观察),采用高通量从海水、滩涂泥壤以及滨海植物分离筛选了324株真菌菌株,在pH 9.4的平板上筛选获得了48个候选产脂肪酶菌株;经ITS序列分析,初步鉴定了其中的12个菌株,分别为镰刀菌、青霉属、枝孢菌、交链孢属等,其中多数为枝孢属和青霉属;进一步利用氢氧化钠滴定法检测其中2个菌株(#89和#260)培养24 h的发酵液,#89菌株产生的脂肪酶酶活为(6.92±2.40)U·mL^(-1),#260菌株产生的脂肪酶酶活为(7.67±1.70)U·mL^(-1)。In order to explore more lipase-producing strain resources,324 fungal strains were isolated and screened from the plants in seawater,coastal wetland&mud soil and coastal areas by high throughput through the olive oil plate detection(transparent circle observation).Forty-eight candidate lipase-producing strains were screened on the plate with pH 9.4.Through the ITS sequence analysis,12 strains were preliminarily identified as Fusarium,Penicillium,Cladosporium,Alternaria,etc.,most of which were Cladosporium and Penicillium.The fermentation broth of the two strains(#89 and#260)cultured for 24 h was further detected by using the titration method of sodium hydroxide.The lipase activity produced by#89 strain was(6.92±2.40)U·mL^(-1),and the lipase activity produced by#260 strain was(7.67±1.70)U·mL^(-1).

关 键 词：脂肪酶 菌株资源 ITS序列 筛选 

分 类 号：Q936[生物学—微生物学]